钛离子注入对油茶种子生理生化反应及初期生长的影响

为了开辟离子束生物技术在油茶育种研究方面的新途径，本研究采用不同剂量的钛离子注入油茶种子，研究钛离子注入之后油茶种子体内过氧化物酶（POD）、超氧化物歧化酶(SOD)、电解质外渗率的变化，跟踪观察处理油茶种子播种之后的生长状况。结果表明，钛离子注入剂量在6×1016~12×1016 Ti2+/cm2之间时，POD和SOD活性达到峰值，此时电解质外渗率最低。未出芽油茶种子较出芽油茶种子耐受能力更强，受钛离子注入负面影响相对较小。播种结果显示，注入剂量低于12×1016 Ti2+/cm2时，处理油茶种子成活率基本能得到保障，在中等剂量作用下还有促进生长的作用。

Effects of the Ti2+ Ion Implantation on Physiological-biochemical Reaction and Seminal Growth of Camellia oleifera Seeds

The research aimed to provide new approach for study on Camellia oleifera breeding through ion implantation biotechnology.After different doses of titanium ion were implanted into Camellia oleifera seeds,peroxidase（POD）,superoxide dismutase（SOD） and electrolyte leakage rate were researched.The seminal growth state of treated seeds was also studied.The results showed that POD and SOD activity reached the peak and electrolyte leakage rate was lowest while Ti2＋ ion implantation dose between 6×10 16 and 12×10 16 Ti2＋ /cm 2.The resistance of no-sprouting Camellia oleifera seeds was stronger than that of sprouting seeds,so they could be influenced by Ti2＋ ion implantation less.Sowing result showed that the survival rate of treated seeds could be guaranteed while the implanted doses were lower than 12×10 16 Ti 2 ＋ /cm 2.The moderate doses could promote growth.