| 内生真菌HND5分子鉴定及其生物学特性研究 |
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| 引用本文： | 刘先宝,郭志凯,时涛,王永壮,霍姗姗,黄贵修.内生真菌HND5分子鉴定及其生物学特性研究[J].中国农学通报,2011,27(27):272-276. |
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| 作者姓名： | 刘先宝 郭志凯 时涛 王永壮 霍姗姗 黄贵修 |
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| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所/农业部热带农林有害生物入侵监测与控制重点实验室,海南儋州,571737
2. 热作两院种苗组培中心,海南儋州,571737 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项"内生拮抗真菌HND5防治香蕉根结线虫研究"，科技部科技人员服务企业项目"香蕉健康种苗标准化生产技术的应用及推广"，中国热带农业科学院博士启动经费"未培养香蕉内生细菌类群多态性分析及其功能验证" |
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| 摘 要： | HND5具有内生性并且对多种病原真菌有拮抗作用，是一株很有应用潜力的生防菌株。本研究对该菌株核糖体内转录间隔区和β微管蛋白基因内转录间隔区进行了分析，其序列和紧密枝顶孢同源性最高。生物学特性研究表明其生长最适培养基为PDA，最适温度为28℃，最适pH为7，最适碳氮源分别为蔗糖和硝酸钠，菌株对重铬酸钾具有耐受性。
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| 关 键 词： | 阳离子盐 阳离子盐 奶牛 生产性能 离子平衡 |
| 收稿时间： | 2010/8/18 0:00:00 |
| 修稿时间： | 2010/9/29 0:00:00 |
Molecular Identification and Biological Characteristics of Endophytic HND5 |
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| Liu Xianbao,Guo Zhikai,Shi Tao,Wang Yongzhuang,Huo Shanshan,Huang GuiXiu.Molecular Identification and Biological Characteristics of Endophytic HND5[J].Chinese Agricultural Science Bulletin,2011,27(27):272-276. |
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| Authors: | Liu Xianbao Guo Zhikai Shi Tao Wang Yongzhuang Huo Shanshan Huang GuiXiu |
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| Institution: | Liu Xianbao 1,Guo Zhikai 1,Shi Tao 1,Wang Yongzhuang 2,Huo Shanshan 1,Huang GuiXiu 1(1 Key Laboratory of Monitoring and Control of Tropical Agriculture and Forest Invasive Alien Pests/Ministry of Agriculture,Environment and Plant Protection Institute,CATAS,Danzhou Hainan 571737,2 Seedling Tissue Culture Center of CATAS and SCUTA,Danzhou Hainan 571737) |
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| Abstract: | HND5 is an endophytic fungi and had antagonistic activity on varieties of pathogenic fungi,so it had application potential for biocontrol.Sequences analysis of ribosomal DNA and β-tubulin internal transcribed spacer region show HND5 had the highest homologous with Acremonium strictum.Biological characteristics research showed the optimum medium was PDA,the optimum temperature was 28℃,and the optimum pH was 7.The optimum carbon and nitrogen source were cane sugar and NaNO 3,respectively.HND5 had the tolerance for potassium dichromate. |
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| Keywords: | HND5 molecular identification biological characteristic |
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