| CHE饲料发酵过程中淀粉酶活力的在线监测 |
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| 引用本文： | 任健,童应凯,黄亮,王斌.CHE饲料发酵过程中淀粉酶活力的在线监测[J].中国农学通报,2011,27(26):35-38. |
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| 作者姓名： | 任健 童应凯 黄亮 王斌 |
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| 作者单位： | 天津农学院,天津300384,天津市祥华生物饲料科技有限公司 |
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| 基金项目： | 中华人民共和国科学技术部天津市科学技术委员会-科技人员服务企业行动项目"生物饲料-CHE研发与产业化" |
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| 摘 要： | 淀粉酶（包括α-淀粉酶和β-淀粉酶）是饲料复合酶制剂中最主要的酶之一。本文对CHE饲料中的α-淀粉酶及淀粉酶全酶进行了提取并进行了活性测定。结果表明α-淀粉酶和β-淀粉酶的最适温度分别为52℃和45℃。α-淀粉酶酶活力最大为5.6352ug/min， β-淀粉酶酶活力最大为1.0931ug/min。
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| 关 键 词： | 内源激素 内源激素 |
| 收稿时间： | 2011/2/15 0:00:00 |
| 修稿时间： | 2011/3/28 0:00:00 |
The Enzymes Assay Of The Fermentation Of CHE Feed On Line |
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| Ren Jian,Tong Yingkai,Huang Liang,Wang Bin.The Enzymes Assay Of The Fermentation Of CHE Feed On Line[J].Chinese Agricultural Science Bulletin,2011,27(26):35-38. |
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| Authors: | Ren Jian Tong Yingkai Huang Liang Wang Bin |
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| Institution: | Ren Jian,Tong Yingkai,Huang Liang,Wang Bin (Tianjin Agricultural University,Tianjin 300384) |
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| Abstract: | Amylase （including α-amylase and β-amylase） is the one of the most important enzymes in the feed compound enzymes.In this paper,α-amylase and amylase of the CHE feed were extracted and the assay of their activity were carried out.The results showed that optimum temperature of α-amylase was 52 ℃ and the maximum of alkaline protease was 5.6352 μg/min and β-amylase was 45 ℃ and the maximum of alkaline protease was 1.0931 μg/min. |
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| Keywords: | amylase CHE feed 3 5-dinitrosalicylic acid method enzymatic activity |
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