大花萱草不定芽直接诱导和植株再生的研究

本研究旨在不通过愈伤途径，直接诱导大花萱草不定芽，并建立相应的植株再生技术体系。实验以大花萱草‘32-1’幼芽为外植体，配制不同激素和活性炭的培养基，考察各因素对大花萱草植株再生的影响。结果表明：不同激素配比对大花萱草‘32-1’初代培养、继代增殖和生根培养影响差异显著。最适初代培养基为MS+6-BA 3.0 mg/L+NAA 2.0 mg/L，启动率和增殖系数分别是100.00%和5.60，可直接诱导出不定芽；最适增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L，增殖系数为3.47；最佳生根培养基为1/2MS+NAA 1 mg/L+AC 2 g/L。

Hemerocallis fulva: Direct Induction of Adventitious Bud and Plant Regeneration

The purpose of this study is to induce adventitious buds directly from young shoots rather than callus, and establish the regeneration system of Hemerocallis fulva. The young shoots of Hemerocallis fulva‘32- 1’were used as explants, the culture medium with different hormones and activated carbon was prepared. The effects of every factor on plant regeneration of Hemerocallis fulva were studied. The results showed that different matching of hormones had significant difference on primary culture, multiplication and rooting culture of Hemerocallis fulva‘32-1’. The optimum initiation medium was MS+6-BA 3.0 mg/L+NAA 2.0 mg/L, the initiation rate and proliferation coefficient was 100.00% and 5.60, respectively, and this medium could induce the adventitious buds directly. The optimal multiplication medium was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L, and the multiplication coefficient was 3.47. The appropriate rooting medium was 1/2MS+NAA 1 mg/L+AC 2 g/L.