杨树叶片高效离体再生系统的构建

利用速生杨树（Populus.tomentosa Car）叶片为外植体建立离体培养体系，结果表明，叶脉处和叶柄处，极易发生不定芽；芽大多是从叶片上直接产生；培养基中BA浓度是影响不定芽形成的关键；BA浓度在05~2.0mg/L范围内均有不定芽发生，BA 1.0mg/L与NAA 0.05mg/L搭配有利于不定芽的形成，培养基中添加GA3 0.1~0.2mg/L可以提高不定芽的发生频率，增殖培养基采用MS+BA 0.5mg/L+NAA 0.05mg/L+GA3 0.05mg/L，不仅能够快速增殖，而且芽苗粗壮，玻化率降低。生根过程采用过渡培养效果较好，ABT3生根粉的加入，使试管苗质量大为提高，在此基础上建立起杨树叶片离体再生系统，利用该体系能够获得高的芽苗再生频率，但是不同品种间略有差异。

Sdudy on Efficient in Vitro Regeneration System of Leaves of Populus Tomentosa Carr

The leaves of fast growing poplar were used as explants and a in vitro culture system was established. The results showed that the leaf vein and petiole produced adventitious buds very easily and most buds occurred directly in the leaves; the BA concentration in media is the key to the bud formation; when the concentration was 0.5~2.0mg/L BA, the adventitious buds occurred, and combining 1.0mg/L BA with0.05mg/l NAA was beneficial to the adventitious bud formation; adding 0.1~0.2mg/L GA3 to the media could increase the Frequency of producing the adventitious buds. Use the propagation medium MS 0.5mg/L BA 0.05mg/L NAA 0.05mg/L GA3 could not only realize rapid propagation but also make the plantlets robust and the verifications rate lower. Adopting transition culture in rooting had a better result, and adding ABT3-rooting-powder to the media made the quality of test-tuber plantlets much better. The in vitro regeneration system of leaves of the poplar was established based on the experimental results. Using this system could reach a high regeneration frequency but different varieties would differ a little bit.