| 甜菜M14品系BvM14-UNG基因克隆及生物信息学分析 |
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| 引用本文： | 王盛昊,于冰.甜菜M14品系BvM14-UNG基因克隆及生物信息学分析[J].中国农学通报,2022,38(4):16-22. |
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| 作者姓名： | 王盛昊 于冰 |
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| 作者单位： | 1.黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500;2.黑龙江大学生命科学学院/黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080 |
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| 基金项目： | 中国博士后项目“甜菜M14品系BvM14-STPK互作蛋白鉴定及其耐盐功能研究”(204968)； |
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| 摘 要： | 为研究Bv-UNG蛋白在植物碱基切除修复(base-excision repair,BER)途径中的功能,以甜菜M14品系为材料,根据NCBI上二倍体栽培甜菜UDG编码基因Bv-UNG序列,利用同源序列克隆法获得甜菜M14品系BvM14-UNG基因cDNA全长,并对其进行生物信息学分析.结果 表明,该基因编码374个氨...
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| 关 键 词： | 甜菜M14品系 BvM14-UNG基因 碱基切除修复 基因克隆 生物信息学分析 |
| 收稿时间： | 2021-09-16 |
Cloning and Bioinformatics Analysis of BvM14-UNG Gene in Sugarbeet M14 Line |
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| WANG Shenghao,YU Bing.Cloning and Bioinformatics Analysis of BvM14-UNG Gene in Sugarbeet M14 Line[J].Chinese Agricultural Science Bulletin,2022,38(4):16-22. |
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| Authors: | WANG Shenghao YU Bing |
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| Institution: | 1.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500;2.Key Laboratory of Molecular Biology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080 |
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| Abstract: | To research the function of Bv-UNG protein in base excision repair (BER) pathway, the full-length cDNA of BvM14-UNG gene of sugarbeet M14 line was obtained by homologous sequence cloning method according to the sequence of UDG coding gene Bv-UNG of diploid cultivated sugarbeet on NCBI and its bioinformatics analysis was carried out. The results show that the gene encodes 374 amino acids, the BvM14-UNG protein shows strong hydrophilicity and is a soluble protein, which is mainly composed of random coils and alpha helices, and has water-activated motif, uracil binding motif, minor groove insertion loop motif, uracil glycosylase inhibitor (UGI) binding site and leucine (Leu) site. BvM14-UNG belongs to UDG-F1 family. BvM14-UNG protein is closely related to BvUNG protein, followed by CqUNG protein. This study lays a foundation for the follow-up study on BvM14-UNG enzyme activity of sugar beet M14 line in BER pathway. |
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| Keywords: | sugarbeet M14 line BvM14-UNG gene base-excision repair (BER) gene clone bioinformatics analysis |
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