牦牛转移因子对藏绵羊外周血淋巴细胞转化功能的影响

为研究牦牛转移因子(transfer factor,TF)对藏绵羊外周血淋巴细胞增殖的作用,试验采用MTT法探索不同浓度淋巴细胞、犊牛血清(FCS)、刀豆蛋白A(Concanavalin A,ConA)对淋巴细胞增殖的影响。在最佳条件下,探讨牦牛TF对藏绵羊外周血淋巴细胞增殖的影响。结果表明,当藏绵羊淋巴细胞浓度为5.4×10⁶个/mL,犊牛血清含量为8%,ConA为1 μg/mL时,刺激效果最佳,且TF单独或协同丝裂原时,对淋巴细胞都有促进增殖的效果,且最佳浓度为1.5 mg/mL。体内注射TF后,各剂量组在注射第3天时,淋巴细胞再次受到TF单独或协同ConA刺激时,其增殖效果与细胞组单独增殖效果比较,差异不显著(P>0.05),至第5~7天时,其增殖效果明显高于细胞组,差异显著(P<0.05)。注射2 mL剂量组转化增殖效果优于1 mL和3 mL剂量组,差异显著(P<0.05)。说明牦牛TF能够有效增强机体免疫力,是一种很好的免疫增强剂。

Effects of Yak Transfer Factor on Tibetan Sheep Peripheral Blood Lymphocyte Proliferation

To investigate the effects of yak transfer factor (TF) on the proliferation of peripheral blood lymphocytes in Tibetan sheep, the effects of lymphocytes, fetal calf serum (FCS) and concanavalin A(ConA) of different concentrations on promoting lymphocyte proliferation were tested by MTT test. Under optimal conditions, the effect of yak TF on peripheral blood lymphocyte proliferation in Tibetan sheep was explored. The results of the experiment demonstrated that, under the combination of lymphocyte, ConA and FCS of 5.4×10⁶ unit/mL, 1 μg/mL and 8% respectively, the best stimulation effect could be achieved. Yak TF only or TF with ConA of 1.5 mg/mL (the best concentration) could also promote lymphocyte proliferation. 3 days after yak TF was injected in each dose group, the proliferation effect of sheep lymphocytes under the stimulation of TF only or TF with ConA became stronger, but the difference was not significant compared with the individual proliferation effect of cell groups (P>0.05). The effect was significantly higher than that of the cell groups at 5-7 days after the injection (P<0.05). The conversion proliferation effect of the 2 mL dose group was better than that of the 1 mL and 3 mL groups, and the difference was significant (P<0.05). Yak TF can effectively enhance the immunity.