甘蓝型油菜DH系在不同生态区SPAD值的差异分析

以甘蓝型油菜纯合双单倍体(DH)为材料,研究不同生态环境下油菜叶绿素SPAD值的变化状况,揭示叶绿素含量遗传表现规律,创制更高叶绿素含量种质资源,以期为油菜高光效育种奠定方法和理论基础。选取了170份甘蓝型油菜DH系,连续3年分别种植在冬油菜区(陕西大荔)和春油菜区(甘肃张掖)。结果表明:无论是不同遗传背景的DH系、还是同一亲本下的不同DH系材料,油菜DH系叶绿素含量在田间既表现出遗传稳定性,又容易受到外界环境影响,即在相似的生态条件下,同一区域即便不同年份的各DH系材料,叶绿素SPAD值的表现基本一致,但在不同生态区,油菜DH系的SPAD值差异很大。通过对油菜DH系亲本和DH系叶绿素SPAD值频数分布分析,叶绿素含量连续3年在2个生态环境具有广泛的连续分布,并且服从正态或者近似于正态分布,以及超亲分离的特点。这些都表明油菜叶绿素含量是一个典型的数量性状,受到多对基因的控制。

Differential Analysis of SPAD Value with Doubled Haploid Lines in Brassica napus L. in Different Ecological Environments

The variation of SPAD value with doubled haploid lines in Brassica napus L. was analyzed in different ecological environments, to reveal the genetic regularity of chloroplast content, innovate new germplasm resources with higher chlorophyll content and establish new approach and theory for high photosynthetic efficiency breeding. In this paper, 170 DH lines in B.napus L. were planted in winter rapeseed area (Shaanxi Dali) and in spring rapeseed area (Gansu Zhangye) for three consecutive years. The results showed that the chlorophyll content had the genetic stability and was easily subjected to environment influence in DH lines of different genetic background or different DH lines materials of the same parents, the performance trend of SPAD value was basically the same in the same ecological environment of different years, but had extremely significant difference in different ecological environments. According to analysis of SPAD value frequency distribution for DH lines parents and DH lines, there was a wide segregation range and continuous distribution of chlorophyll content in two ecological environments in three consecutive years, and normal or near-normal distribution with transgressive segregation. These results also indicated chlorophyll content of B.napus L. was a quantitative trait and genetically controlled by many pairs of genes.