| 枇杷ISSR扩增反应体系的优化 |
| |
| 引用本文： | 赵依杰,王江波,张小红,施维属,林航.枇杷ISSR扩增反应体系的优化[J].中国农学通报,2010,26(7):26-30. |
| |
| 作者姓名： | 赵依杰 王江波 张小红 施维属 林航 |
| |
| 作者单位： | 1. 福州市农业科学研究所,福州,350018
2. 福建农林大学园艺学院,福州,350002 |
| |
| 摘 要： | 采用正交设计对影响枇杷ISSR-PCR的模板DNA、dNTPs、Primer、Taq酶及Buffer(含Mg2+)进行优化。试验结果表明:20μL反应体系中,含模板DNA5ng、dNTPs0.25mmol/L、Primer0.25μmol/L、Taq酶0.5U和10×Buffer(含Mg2+)3μL。此外,从95条ISSR引物中筛选出11条具有丰富的多态性位点的引物,同时优化各引物退火温度。
|
| 关 键 词： | 农业补贴政策 农业补贴政策 综合性收入补贴政策 生产性专项补贴政策 |
| 收稿时间： | 2009-11-13 |
| 修稿时间： | 2009-12-10 |
Optimization of ISSR Reaction System for Species of Loquat (Eriobotrya japonica Lindl.cv) |
| |
| Zhao Yijie,Wang Jiangbo,Zhang Xiaohong,Shi Weishu,Lin Hang.Optimization of ISSR Reaction System for Species of Loquat (Eriobotrya japonica Lindl.cv)[J].Chinese Agricultural Science Bulletin,2010,26(7):26-30. |
| |
| Authors: | Zhao Yijie Wang Jiangbo Zhang Xiaohong Shi Weishu Lin Hang |
| |
| Institution: | Zhao Yijie1,Wang Jiangbo2,Zhang Xiaohong1,Shi Weishu2,Lin Hang1 (1Fuzhou Institute of Agricultural Sciences,Fuzhou 350018,2College of Horticulture,Fujian Agriculture , Forestry University,Fuzhou 350002) |
| |
| Abstract: | The factors of template DNA, dNTPs, Primer, Taq polymerase and Buffer （containing Mg2＋）, greatly influencing ISSR-PCR of loquat, were optimized by orthogonal design. The result shows that the optimum amplification condition of ISSR-PCR is DNA template 5 ng, dNTPs 0.25 mmol/L, Primer 0.25 μmol/L,Taq Polymerase 0.5 U and 10×Buffer （contain Mg2＋） 3 μ in 20 μL reaction volumes. Besides, 11 ISSR primers has been obtained with high stability and polymorphism simultaneity obtained for ISSR-PCR annealing temperature from 95 ISSR primers. |
| |
| Keywords: | ISSR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
