| 利用SRAP标记分析彩色棉与白色棉的遗传差异 |
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| 引用本文： | 凌 磊,李廷春,李正鹏,蔡 沂,孙 旭,苏 翔,林 毅,蔡永萍.利用SRAP标记分析彩色棉与白色棉的遗传差异[J].中国农学通报,2009,25(16):32-38. |
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| 作者姓名： | 凌 磊 李廷春 李正鹏 蔡 沂 孙 旭 苏 翔 林 毅 蔡永萍 |
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| 作者单位： | 安徽农业大学生命科学学院,合肥,230036 |
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| 基金项目： | 安徽省教育厅重点项目，安徽省科技攻关项目 |
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| 摘 要： | 利用SRAP分子标记技术对彩色棉遗传多样性进行研究，应用NTSYS软件对30种供试棉花材料的SRAP-PCR结果进行分析，从中筛选得到29对条带清晰的多态性引物，共产生1067条清晰条带，多态性条带132条，平均每个引物组合得到4.55条多态性条带。聚类分析29对引物组合的扩增结果，Jaccard’s相似系数在0.5405-0.9109之间，利用SRAP标记可以判明彩色棉和白色棉的遗传差异，可有效地应用于彩色棉的遗传多样性及亲缘关系的研究。
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| 关 键 词： | 彩色棉 SRAP 遗传多样性 聚类分析 |
| 收稿时间： | 2009-04-09 |
| 修稿时间： | 2009-04-17 |
Genetic Difference Analysis between Colored Cotton and White Cotton by SRAP Markers |
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| Ling Lei,Li Tingchun,Li Zhengpeng,Cai Yi,Sun Xu,Su Xiang,Lin Yi,Cai Yongping.Genetic Difference Analysis between Colored Cotton and White Cotton by SRAP Markers[J].Chinese Agricultural Science Bulletin,2009,25(16):32-38. |
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| Authors: | Ling Lei Li Tingchun Li Zhengpeng Cai Yi Sun Xu Su Xiang Lin Yi Cai Yongping |
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| Institution: | (School of Life Sciences, Anhui Agricultural University, Hefei Anhui 230036) |
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| Abstract: | The genetic diversity of colored cotton was studied by SRAP, and the results of SRAP-PCR of 30 spices of cotton materials were analyzed by NTSYS software. Twenty-nine primer combinations were selected from 88 primer pairs, which amplified 132 polymorphic bands with an average of 4.55 polymorphic bands per primer pair. Cluster analysis by using UPGMA method based on the data of SRAP amplified bands by 29 primer pairs showed that Jaecard's similarity coefficient ranged from 0.5405 to 0.9109. The results indicated that the genetic difference between colored cotton and white cotton can be distinguished by SRAP, and SRAP molecular marker can be applied to study the colored cotton genetic diversity efficiently. |
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| Keywords: | SRAP colored cotton SRAP genetic diversity cluster analysis |
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