| 农杆菌介导的抗菌肽基因SPCEMA对马铃薯的遗传转化 |
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| 作者姓名： | limy@swau.cq.cn |
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| 作者单位： | 1. 西南大学园艺园林学院,重庆,400716
2. 西南大学农业部蚕桑学重点实验室,重庆,400716 |
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| 基金项目： | 重庆市科委生物技术攻关项目“马铃薯抗晚疫病的基因工程”资助[批准文号：渝科计（1998）18]. |
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| 摘 要： | (1西南大学园艺园林学院，重庆 400716；2西南大学农业部蚕桑学重点实验室，重庆 400716)
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| 关 键 词： | 湖南省山茶属红山茶组植物 湖南省山茶属红山茶组植物 园林观赏特性 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-112005-12-08 |
The Introduction of SPCEMA Gene into Potato (solanum tuberosum L.) by Agrobacterium tumefaciens |
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| limy@swau.cq.cn.The Introduction of SPCEMA Gene into Potato (solanum tuberosum L.) by Agrobacterium tumefaciens[J].Chinese Agricultural Science Bulletin,2006,22(5):63-63. |
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| Authors: | Yang Lirong Li Mingyang Zhu Qinlong Xu Li |
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| Institution: | 1 College of Horticulture and Landscape Engineering, Southwest University, Chongqing 400716; 2 The Key Sericultural Laboratory of Agriculture of Ministry, Southwest University, Chongqing 400716 |
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| Abstract: | In order to search an antibacterial potato (Solanum tuberosum L.), Various conditions for potato transformation with Agrobacterium tumefaciens LBA4404 were investigated and optimal conditions for the cultivar " Taiwanhong" were founded. At the same time, an efficient transformation system was developed. 1)The D600 of the Agrobacterium suspension was adjusted to 0.5~0.8 and then stems and leaf discs were dropped into the suspension for 5 min. 2)Stems and leaves were pre-cultured for 4 days and 8 days respectively. 3)The duration of co-culture is 3 days. Through this system SPCEMA gene was introduced into "Taiwanhong" and this was confirmed by PCR and Southern blotting. The transformation frequency of the stems and leaves was 10.00% and 5.79% respectively. |
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| Keywords: | Potato Agrobacterium tumefaciens Transformation SPCEMA |
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