| 抗寒剂的叶喷施用方法及其对水稻秧苗抗寒性的影响 |
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| 引用本文： | 任勃,王翰盈,付婷钰,文祝友,熊海蓉,张先文.抗寒剂的叶喷施用方法及其对水稻秧苗抗寒性的影响[J].中国农学通报,2020,36(33):1-6. |
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| 作者姓名： | 任勃 王翰盈 付婷钰 文祝友 熊海蓉 张先文 |
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| 作者单位： | 1.湖南农业大学资源环境学院,长沙 410128;2.湖南农业大学生物科学技术学院,长沙 410128;3.湖南农业大学化学与材料科学学院,长沙 410128;4.湖南农业大学动物科学技术学院,长沙 410128 |
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| 基金项目： | 科技部国家重点研发计划项目“双季稻全程机械化丰产节本增效关键技术”(2017YFD0301503);“双季稻病虫草害绿色防控与避灾减损关键技术”(2017YFD0301505);作物种质创新与资源利用重点实验室(省部共建国家重点实验室培育基地)科学基金开放项目“植物源型抗寒剂提高水稻耐冷性状的分子机理研究”(19KFXM07);湖南农业大学校级大学生研究性学习和创新性实验计划项目“水稻TCP21基因在胁迫响应过程中的表达和功能鉴定”(xcx19116) |
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| 摘 要： | 为了探明抗寒剂KHYE-1的叶喷施用方法及其对水稻秧苗抗寒性的影响。以早稻品种‘中嘉早17’为材料,用抗寒剂叶喷水稻秧苗并进行低温胁迫处理,用化学法、生化法测得生化数据。结果表明KHYE-1的最佳叶喷施用方法为叶喷浓度0.5%、叶喷次数为1次/天,连续3天、叶喷用量5.0 g/盒;在低温胁迫下,KHYE-1叶喷处理使秧苗N、P、K含量及叶绿素SPAD值维持较高水平,可溶性糖和脯氨酸分别比CK提高了147.4%和72.0%,过氧化物酶(POD)、过氧化氢酶(CAT)活性分别比CK增加了80.2%、2.63%。其整体效果优于ABA。抗寒剂KHYE-1能有效增强秧苗抵抗低温胁迫的能力。
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| 关 键 词： | 水稻 抗寒剂 叶面喷施 抗寒性 低温胁迫 |
| 收稿时间： | 2020-04-20 |
Cold-resistant Agent: Foliar Spraying Application Method and Its Effect on Cold Resistance of Rice Seedlings |
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| Ren Bo,Wang Hanying,Fu Tingyu,Wen Zhuyou,Xiong Hairong,Zhang Xianwen.Cold-resistant Agent: Foliar Spraying Application Method and Its Effect on Cold Resistance of Rice Seedlings[J].Chinese Agricultural Science Bulletin,2020,36(33):1-6. |
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| Authors: | Ren Bo Wang Hanying Fu Tingyu Wen Zhuyou Xiong Hairong Zhang Xianwen |
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| Institution: | 1.College of Resources & Environment, Hunan Agricultural University, Changsha 410128;2.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128;3.Chemistry and Materials Science, Hunan Agricultural University, Changsha 410128;4.College of Animal and Technology, Hunan Agricultural University, Changsha 410128 |
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| Abstract: | The purpose is to study the foliar spraying application method of cold-resistant agent KHYE-1 and its effect on cold resistance of rice seedlings. The early rice variety ‘Zhongjiazao 17’ was used as material, the seedlings were treated with cold-resistant agent and cold stress, and the biochemical indexes of seedlings were further detected with chemical and biochemical method. The results showed that the best application method of KHYE-1 was spray concentration of 0.5%, one time per day for 3 days and spray dosage of 5.0 g/box. Compared with CK, the content of N (Nitrogen), P (Phosphorus), K (Potassium) and the value of chlorophyll SPAD in rice seedlings treated with cold-resistant agent under cold stress maintained at a relatively high level, and the content of soluble sugar and proline increased by 147.4% and 72.0%, and the activities of peroxidase (POD) and catalase (CAT) were 80.2% and 2.63% higher, respectively. In conclusion, the overall effect of KHYE-1 is better than that of ABA, and it could improve the cold-resistance of rice seedlings. |
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| Keywords: | rice cold-resistant agent foliar spraying cold resistance cold stress |
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