利用氦氖激光诱变提高枯草芽孢杆菌纤溶酶活力的研究

通过诱变筛选纤溶酶活性高的枯草芽孢杆菌菌株,得到突变株HDBF-N7HN5。使用氦氖激光诱变,设置不同的诱变时间,通过不同的蛋白平板处理,筛选高产突变株并检测纤溶酶活力。实验结果表明,在氦氖激光诱变处理45 min后,最高产突变株纤溶酶活力达到429.89±5.74 IU/mL。突变株经过10次传代培养后,保持稳定的高产纤溶酶特性。纤溶酶粗酶液处理血凝块的绝对溶解率可达到57.74±0.72%,10倍稀释液处理血凝块的绝对溶解率也能达到26.89±0.68%,菌株产生的纤溶酶具有良好的体外溶栓效果。本研究结果既提高了微生物纤溶酶活性,也为该菌株产纤溶酶的产业化提供了依据。

He-Ne Laser Mutagenesis Increasing the Fibrinolytic Enzyme Activity of Bacillus subtilis

The mutant strain HDBF-N7HN5 was obtained by inducing and selecting Bacillus subtilis strain with high fibrinolytic enzyme. He-Ne Laser mutagenesis was conducted in this study with different mutant time. The mutant strains were selected and the activities of fibrinolytic enzyme were detected with different fibrin plates. The results showed that after 45 min with He-Ne Laser mutagenesis, the fibrinolytic enzyme activity of the mutant strain with the highest yield reached 429.89±5.74 IU/mL. The genetic stability analysis of HDBF-N7HN5 indicated that the production of fibrinolytic enzyme was very stable after 10 generation cultivation. The absolute dissolution rate of blood clot treated with crude fibrinolytic solution reached 57.74±0.72%, and the absolute dissolution rate of blood clot treated with 10-fold dilution could also reach 26.89±0.68%. The fibrinolytic enzyme produced by the strain had good thrombolytic effect in vitro. The results of this study not only increase the microbial fibrinolytic activity, but also provide a basis for the industrialization of fibrinolytic produced by this strain.