芽孢杆菌BJ-6产抗菌物质发酵条件初探

芽孢杆菌（Bacillus sp.）BJ-6分离自北京市门头沟区杏树根围土壤，它能够产生对多种植物病原真菌有强烈拮抗作用的抗菌物质。采用单因素试验和正交试验进行了发酵条件的筛选，发现：芽孢杆菌BJ-6产生抗菌物质的最适液体培养基配方为麦芽糖20g，蛋白胨10g，麸皮10g，CaCO3 10g，Na2HPO3 2g，KH2PO3 1g 和水1000ml；最适发酵条件为：接种种龄10 h、接种量1%、发酵温度30℃~35℃、转速为210 r/min、培养时间60 h和起始pH为9。

Fermentation Conditions for Production of Antifungal substances by Bacillus sp. BJ-6

The strain BJ-6 of Bacillus sp. was isolated from the rhizosphere of apricot trees in Mentougou, Beijing, China, and showed strong antifungal activity to several species of plant pathogenic fungi. The fermentation medium and conditions for antifungal substances from the strain of BJ-6 were optimized by single factor experiments and orthogonal experiments. The results showed that optimum fermentation medium for antifungal substances formula was maltose 20g, peptone 100g, bran10 g,CaCO3 10g, Na2HPO3 2g ,KH2PO3 1g, and water 1000 ml. The favorable fermentation condition was determined：the age of inoculum10 h , inoculation amount 1%,fermentation temperature 30℃~35℃, rotate speed 210 r/min, fermentation time 60 h, and initial pH value of medium 9，.