聚合草根蛋白质氨基酸的测定与评价

采用反相高效液相色谱法测定了聚合草sympHytum pezegrinum L.]根的氨基酸含量，用国际上通用的营养评价方法对聚合草根进行了蛋白质营养价值评价。结果为，聚合草根蛋白质含量11.81%，氨基酸含量3.45%，其中必需氨基酸占39.5%。第一限制氨基酸是蛋氨酸。聚合草根蛋白质的化学评分 (CS)、氨基酸评价(AAS)、必需氨基酸指数(EAAI)、生物价(BV)、营养指数(NI)和氨基酸比值系数(SRCAA)分别为 41.9，42.5，74.3，69.3，8.77和55.24。聚合草根蛋白质的营养价值高于淮山药，低于马铃薯。呈味氨基酸含量占总氨基酸的42.03%，有较好的适口性和较高的开发利用价值。

Determination and Assessment of Amino acid in the Root Protein of SympHytum pezegrinum

A reversed-phase high performance liquid chromatographic method was developed for determining amino acids in the root protein, and internationally adopted nutrient value assessment method was applied to the overall assessment of the protein nutrient value .The results showed the crude protein content was 11.81%, amino acids content was 3.45%, and the total essential amino acid accounted for 39.5% of the total amino acid.Methionine was the first limited amino acid.Chemical score(CS), amino acid score(AAS), essential amino acid index(EAAI), bio1ogical value(BV), nutritional index(NI)and score of ratio coefficient of amino acid(SRCAA) of its protein were 41.9, 42.5, 74.3, 69.3, 8.77 and 55.24 respectively.Compared with two other kinds of root crops,root protein nutrient value of SympHytum pezegrinum was higher than Rhizoma discoreae, and lower than potato.Additionally, the ratio of total delicious amino acid content to total amino acid content(DAA/TAA) was 42.03%, showed its good palatability and high application.