新型不育胞质在栽培稻中的分布与鉴定

为了分析只含有不育基因orf216在栽培稻中的分布，鉴定具有新的细胞质雄性不育胞质类型的种质资源。选取栽培稻824个品系为研究对象，以细胞质雄性不育相关序列HL-Sp1的特异引物进行PCR扩增，初步筛选得到9个候选品系，利用细胞质雄性不育基因orfH79，orf216的特异引物进行PCR扩增完成进一步筛选，并用Southern杂交验证。结果表明，最终筛选出IR72892，IR43，IR735463个只含有不育基因orf216不含orfH79的新型细胞质雄性不育胞质类型的品系。通过分子标记辅助选择，已成功的从824个栽培稻种中筛选到新的具有细胞质雄性不育胞质类型的新品系，为后续不育系的培育和相关理论研究奠定基础。

Distribution and Identification of a New Cytoplasmic Male Sterile (CMS) in Cultivated Rice

Objective] To analyze the distribution of cytoplasmic male sterile (CMS) gene orf216 in cultivate rice, and to identify new resource of cytoplasmic male sterile (CMS). Methods] 824 cultivate varieties were analyzed with PCR amplification using special primer Sp1F/Sp1R of HL-sp1.The results showed that there were 9 accessions with HL-sp1. Then, the above 9 accessions were further analyzed by PCR amplification using CMS related gene orfH79, orf216 and Southern Blot. Results] We found three accessions (IR72892, IR43, IR73546) contained only orf216 no orfH79 in selected cultivate varieties. Conclusion] By marker-assisted selection (MAS) method, new cytoplasmic types related cytoplasmic male sterile (CMS) have achieved from 824 cultivate varieties. These results contribute to the study of gene function and the development of cytoplasmic male sterile (CMS) line in rice in the future.