不同类型供体细胞对牛体细胞重构胚发育能力的影响

(1西北农林科技大学动物科技学院，陕西杨陵 712100；2陕西省畜牧兽医总站，陕西西安710016)

Effect of Type of The Donor Cell on The Development of Bovine Somatic Cell Nuclear Transplantation Reconstituted Embroys

We studied the type of donor cell on the development of bovine somatic cell nuclear transplantation reconstituted embryos. The oocyte was cultured for 22~23h to reach maturity in vitro. The nuclear of the mature oocyte was taken out by micromanipulation. The donor nuclei came from fibroblasts and granular cells. Cells were divided into two halves, which were respectively treated, by serum starvation and non- starvation. The donor nuclei were transplanted into the enucleated oocyte by the microinjection of cytoplasm to form reconstituted embryos. After being activated in Ionomycin media for 5 min, the recombinant embryos were firstly put into 6-DMAP media to be cultured for 4 h, then into the CR1 media. The cleavage rate of the reconstituted embryos was examined after 24h and 36h and the growth rate of the blastocyst was counted after 8 d. The results show that the development rate of blastocyst of the somatic cell treated by serum starvation is strikingly higher than that of the reconstituted embryos from bovine somatic cell treated by serum starvation.(fibroblasts: 3.16% vs 11.76%, p<0.05; granular cells: 7.14% vs 14.96%, p<0.05).There is not much difference in the development rate of blastocyst between the reconstituted embryos from fibroblasts and granular cells. The result implies that the reconstituted embryos from non- starvation treated somatic cells have higher development rate and the embryos from bovine granular cells have higher development rate in vitro.