| 不同稀释剂对猪冷冻精液的影响 |
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| 引用本文： | 江中良,李青旺,胡建宏,王立强,于永生,夏 凡.不同稀释剂对猪冷冻精液的影响[J].中国农学通报,2004,20(4):12-12. |
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| 作者姓名： | 江中良 李青旺 胡建宏 王立强 于永生 夏 凡 |
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| 作者单位： | 西北农林科技大学动物科技学院 陕西杨凌712100
(江中良，李青旺，胡建宏，王立强，于永生)，西北农林科技大学动物科技学院 陕西杨凌712100(夏凡) |
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| 基金项目： | 陕西省科技攻关计划项目(4130303). |
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| 摘 要： | 手握法采集12月龄大约克种公猪精液，在37 ℃下离心，弃去精清，收集浓缩段富含精子部分，用7种不同的稀释液进行稀释，应用液氮熏蒸法制作颗粒冻精。结果表明，在7种稀释液中，7号稀释液 优于其他6种稀释液(p<0.05)；与其他的冷冻保护剂相比，甘油的冷冻保护性能较好(p<0.01)，其适宜浓度为2~4 ml·l-1；干解冻（40~45 ℃）效果优于湿解冻，解冻后精子活率达0.46~0.49（p<0.05）；稀释液中添加安钠咖可有效地延长精子的冻后存活时间(p<0.01)。
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| 关 键 词： | 猪 冷冻精液 冷冻保护剂 冷冻曲线 精子活率 |
Study on the Technique of Boar Semen Freezing |
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| JIANG Zhong-liang,LI Qing-wang,HU Jian-hong,WANG Li-qiang,YU Yong-sheng,XIA Fan.Study on the Technique of Boar Semen Freezing[J].Chinese Agricultural Science Bulletin,2004,20(4):12-12. |
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| Authors: | JIANG Zhong-liang LI Qing-wang HU Jian-hong WANG Li-qiang YU Yong-sheng XIA Fan |
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| Institution: | (College of Animal Science and Technology,Northwest Sci-Tech University of Agriculture and Forestry, Yangling,Shaanxi 712100,China) |
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| Abstract: | The extenders and the cryoprotectant contents of frozen semen pellets were studied using the plate in boar. The results showed that the number 7 was the best extender (p<0.05)in seven groups, and the best cryoprotectant was glycerol (p<0.01).When the glycerol content was 2 percent (p<0.05),the sperm motility of frozen semen pellets was best in all treatment groups.The method of dry thawn of frozen semen pellets was better than that of wet thawn(p<0.05); the sperm motility was 0.46-0.49 after the frozen semen pellet was thawed at 40 to 45 ℃. supplyment in the extender could increase the thawn sperm survival time and motility effectively(p<0.01). |
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| Keywords: | Boar Frozen semen Frozen curve Cryoprotectant Sperm motility |
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