不同激素浓度对橡胶草不同器官直接分化再生苗的影响研究 |
| |
引用本文: | 朱金霞,张源沛,郑国保,孔德杰.不同激素浓度对橡胶草不同器官直接分化再生苗的影响研究[J].中国农学通报,2016,32(31):108-114. |
| |
作者姓名: | 朱金霞 张源沛 郑国保 孔德杰 |
| |
作者单位: | 宁夏农林科学院农业生物技术研究中心,宁夏农林科学院农业生物技术研究中心,宁夏农林科学院农业生物技术研究中心,宁夏农林科学院农业生物技术研究中心 |
| |
基金项目: | 国家科技部国际合作项目“橡胶草种质驯化及提取技术引进”(2014DFR31020);宁夏回族自治区科技支撑对外合作项目“橡胶草种质驯化 及提取技术引进”。 |
| |
摘 要: | 分别以橡胶草(Taraxacum kok-saghyz Rodin)叶片、叶柄、根段为外植体,以MS为基本培养基,比较不同激素浓度对橡胶草不同器官直接分化再生苗的影响。结果表明:橡胶草不同器官直接分化再生苗所需最佳激素浓度不同。(1)最适合诱导叶片和叶柄直接芽分化再生苗的激素浓度均为NAA 0.2 mg/L 6-BA 0.5 mg/L,其芽分化率和每外植体平均芽数分别为36.44%、38.83个和95.83%、10.56个;(2)最适合诱导根段直接芽分化再生苗的激素浓度为NAA 1.0 mg/L 6-BA 0.1 mg/L,其芽分化率和每外植体平均芽数分别为92.25%和21.49个。该研究为橡胶草高频再生体系的建立及进一步利用基因工程进行品种改良奠定了基础。
|
关 键 词: | 大型海藻 大型海藻 鼠尾藻 羊栖菜 溶解氧 产氧速率 耗氧速率 |
收稿时间: | 3/4/2016 12:00:00 AM |
修稿时间: | 2016/4/14 0:00:00 |
Direct Differentiation Regeneration Plants from Different Organs of Taraxacum kok-saghyz Rodin: The Effect of Hormone Concentrations |
| |
Abstract: | In order to study the differentiation abilities of regeneration plants of different organs under different hormone concentrations, vitro leaves, petiole and root segment of Taraxacum kok-saghyz Rodin were used as explants and MS as basic culture medium. The results showed that: the best hormone concentrations for bud induction of different organs of Taraxacum kok-saghyz Rodin were different, (1) NAA 0.2 mg/L 6-BA 0.5 mg/L was most suitable for regeneration by bud induction of leaf and petiole, and the differentiation rate and the number of the average bud per explant were 36.44%, 38.83, 95.83%, 10.56, respectively; (2) NAA 1.0 mg/L 6- BA 0.1 mg/L was most suitable for regeneration by bud induction of root, the differentiation rate and the number of the average bud per explant were 95.25% and 21.49, respectively. The results lay a foundation for the establishment of a high frequency regeneration system and further variety improvement by using gene engineering technology. |
| |
Keywords: | Taraxacum kok-saghyz Rodin organs direct differentiation regeneration plants |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |