药用植物红落藜组织培养研究

本试验以红落藜为试验材料，进行茎段组织培养及植株再生的研究。试验结果表明75%酒精5s+0.1%升汞8min既可进行彻底消毒，又保证具有较高的成活率；通过初代培养筛选出NAA为最适的生长激素，最适培养基为MS + 6-BA 1.5 mg?L-1 + NAA 0.2mg?L-1+琼脂0.8%+蔗糖2%+活性炭0.2%；通过继代培养筛选出最适培养基为MS + 6-BA 2.0 mg?L-1 + NAA 0.2mg?L-1+琼脂0.8%+蔗糖2%+活性炭0.2%；最适的生根培养基为1/2MS + IBA1.0 mg?L-1 + NAA 0.1mg?L-1+琼脂0.8% + 蔗糖2% + 活性炭0.2%，生根率为80%。当试管苗叶片数3～5片，苗高2cm～3cm，生根数3～4条时，移栽至蛭石和腐殖土(1∶2)混合的基质中，保湿遮阴，成活率可达90％以上。

Studies on Plantlet Regeneration and Propagation of Chenopodium album

The trial used the stems of Chenopodium album L. for the tissue culture. With MS as the basic medi- um, after disinfection method, the proliferation of media, rooting medium screening, the results showed that 75% alcohol 5 s ＋0.1% mercuric chloride 8 rain to conduct a thorough disinfection, and ensure a higher survival rate ;MS＋6-BA 1.5 mg/L＋ NAA 0.2 mg/L＋ agar 0.8% ＋ sucrose 2% ＋ activated charcoal 0.2% for primary culture, MS ＋6-BA 2.0 mg/L＋ NAA 0.2 mg/L＋ agar 0.8% ＋ sucrose 2% ＋ activated charcoal 0.2% for subcul- ture, 1 / 2MS＋IBA 1.0 mg/L＋ NAA 0.1 mg/L＋ agar 0.8% ＋ sucrose 2% ＋ activated charcoal 0.2% for the grow- ing of roots, rooting rate of 80%.When the plantlets with 3 -5 leaves, the height of 2-3 cm and 3 to 4 roots were transferred onto the mixture of lennilite and rotten breed soil（1：2）, and the survival rate was up to 90%.