非淀粉多糖酶对牙鲆消化酶活性和饲料消化率的影响

以初始平均体重(2.04 ± 0.02)g的牙鲆(Paralichthys olivaceus)为实验对象，进行为期70 天的摄食生长实验，研究不同添加方式的非淀粉多糖酶对牙鲆消化酶活性和饲料消化率的影响。在5000.0 g豆粕中添加25.0 g非淀粉多糖酶，然后用产朊假丝酵母(Candida utilis)进行发酵预处理，得到非淀粉多糖酶预处理豆粕。共制作4种等氮等能饲料，其中以全鱼粉饲料为对照饲料，用豆粕蛋白替代45%的鱼粉蛋白配制成豆粕组饲料；在豆粕组饲料中添加0.2%非淀粉多糖酶配制成非淀粉多糖酶组饲料；用非淀粉多糖酶预处理豆粕蛋白替代45%的鱼粉蛋白配制成非淀粉多糖酶预处理豆粕组饲料。结果表明，用豆粕蛋白替代饲料中45%的鱼粉蛋白，若不添加非淀粉多糖酶则显著降低牙鲆肝、肠的消化酶活性(P<0.01)和营养成分的表观消化率(P<0.05)；在含豆粕饲料中添加0.2%非淀粉多糖酶显著提高牙鲆肝、肠的消化酶活性和营养成分的表观消化率(P<0.05)；在饲料中添加非淀粉多糖酶预处理豆粕没有显著提高牙鲆肝、肠的消化酶活性和营养成分的表观消化率(P>0.05)。

Effects of Non-starch Polysaccharide Enzyme on Digestive Enzyme Activity and Apparent Digestibility of Japanese Flounder (Paralichthys olivaceus)

A study was conducted to evaluate the effects of non-starch polysaccharide (NSP) enzyme on digestive enzyme activity and apparent digestibility of Japanese flounder (Paralichthys olivaceus). The NSP-enzyme pretreated soybean meal (SBM) was obtained by fermentation with Candida utilis. Four isonitrogenous and isocaloric diets were formulated. The control diet contained 68.0% fish meal (FM) as the main protein source, whereas 45% of FM protein was replaced by SBM protein in the SBM diets with 0 and 0.2% NSP-enzyme. The NSP-enzyme pretreated SBM diet was formulated in which NSP-enzyme pretreated SBM protein replaced 45% of FM protein. At the end of the feeding trial, the digestive enzyme activities (P<0.01) and apparent digestibility (P<0.05) of fish fed the SBM diet without NSP-enzyme were significantly lower than those in control. Comparing with the SBM diet without NSP-enzyme, the fish fed the SBM diet supplemented NSP-enzyme showed significantly higher digestive enzyme activities (P<0.01) and apparent digestibility (P<0.05). The digestive enzyme activities and apparent digestibility of fish fed NSP-enzyme pretreated SBM diet were not significantly different from those of the SBM diet without NSP-enzyme (P>0.05).