兔出血症病毒的鉴定及其系统发育分析

为确定临床家兔的死亡病因并对其病原进行研究分析，本试验通过临床症状、剖检变化和RT-PCR技术以及HA-HI试验对一起家兔的慢性死亡进行了诊断。结果表明：为兔出血症病毒感染引起的兔瘟。然后设计引物对该病毒的唯一结构蛋白VP60衣壳蛋白的全基因序列进行了RT-PCR扩增、测序和比对分析。结果显示：引起本次家兔死亡的兔出血症病毒的VP60长度与其他已知毒株一致，也是由1740个碱基组成，与已发表毒株的VP60的同源性达90%以上；在系统进化上与俄罗斯2009年的一个流行毒株同源性最高(98.1%)。本试验研究结果表明本次引起非典型兔瘟的兔出血症病毒仍然具有较高的保守性，但病毒的结构蛋白也发生了一些小的变异，这些变异成为基因亚群划分的依据。

Identification of Rabbit Hemorrhagic Disease Virus and Phylogenetic Analysis

In order to determine and analyze the pathogeny of the clinical rabbits dying after natural infection, diagnosis was done to the rabbits by clinical symptoms, pathological changes and RT-PCR technology, as well as HA-HI test in this experiment. The results showed that the pathogen was rabbit hemorrhagic disease virus (RHDV). Then the complete sequence of capsid protein, VP60, which was the only structural protein of RHDV was amplified by RT-PCR with specific primers and amplification products were directly sequenced, assembled and analyzed. The results showed that the sequence of VP60 of RHDV isolated consisted of 1740 nucleotides just like other known strains. Phylogenetically, the sequence shared homology of more than 90% with worldwide isolates among RHDV strains and the highest homology of 98.1% with an isolate from Russia of 2009. From the study, we could saw that the virus isolated from untypical rabbit plague still had high conservation. However, there were also some minor variations in the structural protein, which was the basis of the classification of gene groups.