蟾酥注射液对小鼠免疫细胞影响的实验研究

为了观察蟾酥注射液对小鼠的免疫细胞增殖及其分泌细胞因子的作用,采用中药免疫药理学方法,选择不同浓度的蟾酥注射液作为试验组,分别与小鼠的脾淋巴细胞以及LPS或者ConA共同孵育48 h,检测细胞增殖水平的变化;将蟾酥注射液与小鼠腹腔巨噬细胞共培养,检测蟾酥注射液对巨噬细胞吞噬中性红能力的影响;将蟾酥注射液与自然杀伤细胞共培养,检测蟾酥注射液对NK细胞杀伤能力的影响;取脾细胞与蟾酥注射液共培养上清,ELSIA法检测蟾酥注射液对几种重要的细胞因子（IFN-γ、IL-2、IL-12）分泌水平的变化。结果显示蟾酥注射液在一定剂量范围内单独或者协同非特异性丝裂原（Con A或LPS）作用能够显著增强小鼠脾淋巴细胞的增殖能力（P〈0.05或P〈0.01）;蟾酥注射液单独作用可以显著提高巨噬细胞吞噬中性红的能力;蟾酥注射液能够显著提高小鼠NK细胞对靶细胞的杀伤活性;同时蟾酥注射液能够显著增强小鼠脾淋巴细胞分泌Th1型细胞因子。说明蟾酥注射液在体外能够显著提高几种主要免疫细胞的增殖和功能,研究结果显示蟾酥注射具有显著的非特异性免疫增强作用。

Research on Bufonis Parenteral Effects on Mice Immunocytes

In the present study, we observed the effects of bufonis parenteral solution on in vitro immunocytes proliferation and three kind of cytokines secretion. Methods： We choose the traditional Chinese immunopharmacology method to test the effect of bufonis parenteral solution on the immunocytes. We take the Balb/c mouse spleen lymphocyte incubate with bufonis parenteral solution combine with LPS or Con A, for test spleen proliferation activity; bufonis parenteral solution incubate with peritoneal macrophage for test the capability of phagocytosis; bufonis parenteral solution incubate with NK cell, for test the cytotoxic activity of Yac-1 cells. We also evaluated spleen lymphocyte secrete cytokines by ELISA method. The results showed that bufonis parenteral solution treated alone or combined with polyclonal T cell mitogen （Con A or LPS） in a certain dose range, the effects significantly enhanced the proliferation, tral red capacity （P〈0.05, P〈 0.01）. Bufonis and the solution significantly promote the PMФ phagocytosis neuparenteral solution alone significantly enhanced the NK cytotoxic activity of Yac-1 cells. Bufonis parenteral solution also induce cytokines secretion in vitro. The results indicated that bufonis parenteral solution not only enhances adaptive immunity, but also activates innate immunity.