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| ITS序列在苦荞麦种质资源鉴定中的应用 | |
| 引用本文： | 邵美红,孙加炎,林兵,秦路平.ITS序列在苦荞麦种质资源鉴定中的应用[J].中国农学通报,2012,28(6):131-134. |
| 作者姓名： | 邵美红 孙加炎 林兵 秦路平 |
| 作者单位： | 1. 浙江省建德市种子管理站,浙江建德,311600 2. 浙江省种子总站,杭州,310020 3. 第二军医大学药学院,上海,200433 |
| 基金项目： | 浙江省种子工程项目（浙农计[2009]168号） |
| 摘 要： | 为了更精确有效地鉴定苦荞麦种质资源，根据ITS序列通用引物，以苦荞麦基因组DNA为模板通过PCR方法扩增出ITS(internal translation spacer)序列，在克隆测序的基础上对荞麦属5个栽培苦荞麦中进行分子鉴定与亲缘关系分析。结果表明：（1）5个供试材料ITS序列的变异小，序列长度在584~590 bp之间，G+C含量高，为67.5%~68.5%。（2）通过聚类方法分析发现‘建德苦荞麦’和‘红花苦荞麦’聚为一支，‘西荞2号’和‘西荞3号’聚为一支。 |
| 关 键 词： | 植物激素 植物激素 预处理技术 分析检测 |
| 收稿时间： | 2011/8/23 0:00:00 |
| 修稿时间： | 2011/10/31 0:00:00 |
Application Research of ITS Sequences in Fagopyrum tataricum Identification |
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| Shao Meihong , Wu Wei , Lin Bing , Sun Jiayan , Qin Luping.Application Research of ITS Sequences in Fagopyrum tataricum Identification[J].Chinese Agricultural Science Bulletin,2012,28(6):131-134. | |
| Authors: | Shao Meihong Wu Wei Lin Bing Sun Jiayan Qin Luping |
| Institution: | 1Zhejiang Jiande Seed Control Station,Jiande Zhejiang 311600;2Seed Management Station of Zhejiang Province,Hangzhou 310020;3Pharmaceutical College,the Second Military Medical University,Shanghai 200433) |
| Abstract: | In order to identify germplasm resources of Fagopyrum tataricum,in this study the ITS general primers of sequence was used,F.tataricum genomic DNA as a template was amplified by PCR out of ITS sequences（internal translation spacer）,the cloned sequence was based on 5 cultivars of F.Tataricum for molecular identification and molecular analysis of genetic relationship.The results showed that：（1） five materials were a small variation of ITS sequences,the length of sequences between 584-590 bp,G＋C content was high up to 67.5%-68.5%.（2） Using different clustering analysis it showed that＇Jiande＇F.Tataricum and ＇Honghua＇F.Tataricum＇,Xi 2＇F.Tataricum and＇Xi 3＇F.Tataricumt had a closed genetic relationship. |
| Keywords: | Fagopyrum tataricum ITS sequences germplasm identification |
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