大豆异黄酮对奶牛脾脏与肠系淋巴结中淋巴细胞增殖的影响

为了研究大豆异黄酮对奶牛脾脏与肠系淋巴结中淋巴细胞增殖与活化的影响，并揭示大豆异黄酮提高奶牛免疫性能的作用机理。试验采用6×2（浓度×时间）实验设计，各浓度梯度的大豆异黄酮 (100.00、25.00、5.00、1.00、0.25、0.05 μg/mL)与淋巴细胞共育培养48 h和60 h后，用酶标仪在570 nm处测得各孔的OD值。结果表明：（1）添加0.05~25 μg/mL大豆异黄酮能够促进奶牛肠系淋巴结中淋巴细胞的增殖，在添加量为5 μg/mL时达到极显著的促进作用，但大豆异黄酮添加剂量达100 μg/mL时显著抑制肠系淋巴结淋巴细胞的增殖；（2）100 μg/mL和25 μg/mL大豆异黄酮添加组极显著抑制奶牛脾脏淋巴细胞的增殖(P<0.01)，而1 μg/mL浓度组则显著促进奶牛脾脏淋巴细胞的增殖(P<0.05)，其余各浓度组对奶牛脾脏淋巴细胞有抑制作用，但差异不显著。

The Effects of Soy Isoflavone on the Lymphocyte Proliferation in Lymph Nodes and Splenocyte from Dairy Cow

To study the effects of Soy Isoflavones on lymphocyte proliferation in spleen and lymph nodes of Holstein cows,investigate the mechanism to improve immune performance of cows.The experiments were assigned into 7 groups：control group （blank）,100.00,25.00,5.00,1.00,0.25,0.05 μg/mL,Soy Isoflavones and incubated with lymphocyte to 48 h or 60 h,respectively.The plates were read at 570 nm.The results showed that：Firstly,for the lymphocyte of lymph nodes,all over the concentrates of Soy Isoflavones ranging from 0.05 μg/mL to 25 μg/mL had promoted effect to the lymphocyte proliferation,while the most promotion concentrates was 5 μg/mL it reach the most promotion effect.But when the addition concentrations of Soy Isoflavones enhanced reached to 100 μg/mL,the inhibition suppressed effect of lymphocyte proliferation was significantly;Second,in lymphocyte of splenocyte,the lymphocyte proliferation at higher concentrations （100 μg/mL and 25 μg/mL Soy Isoflavones group） had a significantly suppressed effect on the proliferation of lymphocyte in the spleen of the cow （P0.01）.（P0.01） comparing with the control.However,at lower concentrations （1 μg/mL） the lymphocyte proliferation could be significantly stimulated （P0.05）.The other groups had the same inhibition suppressed effect,but the difference was not significant.