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| 不同培养基成分对铁皮石斛组织培养的影响 | |
| 引用本文： | 宋顺,许奕,王必尊,刘以道,覃和业,马蔚红.不同培养基成分对铁皮石斛组织培养的影响[J].中国农学通报,2013,29(13):133-139. |
| 作者姓名： | 宋顺 许奕 王必尊 刘以道 覃和业 马蔚红 |
| 作者单位： | 1. 中国热带农业科学院海口实验站，海口570102; 海南省香蕉遗传改良重点实验室，海口570102 2. 热作两院种苗组培中心,海南儋州,571737 3. 中国热带农业科学院海口实验站，海口570102; 海南省香蕉遗传改良重点实验室，海口570102; 热作两院种苗组培中心，海南儋州571737 |
| 基金项目： | 热作两院种苗组培中心企业展基金(201201)。 |
| 摘 要： | 以铁皮石斛无菌苗的茎段作为外植体，讨论MS培养基中添加不同浓度的激素对铁皮石斛的诱导、增殖、分化以及生根的影响。结果表明：最适合铁皮石斛原球茎诱导的培养基为MS+ 6-BA 0.5 mg/L+ NAA 1.5 mg/L，原球茎诱导率为95%；最适合原球茎增殖的培养基为MS+ 6-BA 1 mg/L+ NAA 1 mg/L；最适合原球茎分化的培养基为MS+ 6-BA 5 mg/L+ NAA 1 mg/L，其分化率达80%；最适合铁皮石斛根诱导的培养基为MS+IBA 1.5 mg/L+香蕉泥100 g/L，其生根率为100%。 |
| 关 键 词： | 致病性 致病性 |
| 收稿时间： | 2012/11/5 0:00:00 |
| 修稿时间： | 2012/11/11 0:00:00 |
Different Media on the Tissue Culture of Dendrobium officinale |
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| Song Shun , Xu Yi , Wang Bizun , Liu Yidao , Qin Heye , Ma Weihong.Different Media on the Tissue Culture of Dendrobium officinale[J].Chinese Agricultural Science Bulletin,2013,29(13):133-139. | |
| Authors: | Song Shun Xu Yi Wang Bizun Liu Yidao Qin Heye Ma Weihong |
| Abstract: | Using the stem of the Dendrobium officinale as explants, the effects of the MS medium with different concentrations of hormone on induction, proliferation, differentitation and taking root were studied. The result showed that MS+ 6-BA 0.5 mg/L+ NAA 1.5 mg/L was the suitable medium for the protocorm induction, the rate was 95%; MS+ 6-BA 1 mg/L+ NAA 1 mg/L was the ideal medium for the protocorm proliferation; MS+ 6-BA 5 mg/L+ NAA 1 mg/L was the best medium for the protocorm differentitation, the rate was 80%; MS+IBA 1.5 mg/L+ banana 100 g/L was the right medium for inducing the root growth, the rate was 100%. |
| Keywords: | Dendrobium officinale tissue culture plant hormones |
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