| BVDV基因E0在人参发根的转化及其分子检测 |
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| 引用本文： | 李然 臧,埔 郜玉钢 马,琳 王亚星 李,学 李,萍 张连学.BVDV基因E0在人参发根的转化及其分子检测[J].中国农学通报,2011,27(10):239-242. |
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| 作者姓名： | 李然 臧 埔 郜玉钢 马 琳 王亚星 李 学 李 萍 张连学 |
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| 作者单位： | 吉林农业大学中药材学院,长春,130118 |
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| 基金项目： | 科技部项目，国家科技支撑计划项目，中国博士后科学基金 |
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| 摘 要： | 将重组鹿源BVDV基因E0植物表达载体（pBI121/E0），通过发根农杆菌Ri介导，用叶盘法转化得到人参发根，分别用PCR、RT-PCR检测其转化情况。结果表明：获得了转鹿源BVDV基因E0人参发根，鹿源BVDV基因E0已整合到人参发根基因组中并得到了转录，为研制转基因人参发根疫苗提供了科学依据和材料。
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| 关 键 词： | 抗性评价 抗性评价 |
| 收稿时间： | 2010/11/24 0:00:00 |
| 修稿时间： | 2010/12/29 0:00:00 |
Transformation and Molecular Detection of BVDV Gene E0 in Ginseng Hairy Roots |
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| Li Ran,Zang Pu,Gao Yugang,Ma Lin,Wang Yaxing,Li Xue,Li Ping,Zhang Lianxue.Transformation and Molecular Detection of BVDV Gene E0 in Ginseng Hairy Roots[J].Chinese Agricultural Science Bulletin,2011,27(10):239-242. |
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| Authors: | Li Ran Zang Pu Gao Yugang Ma Lin Wang Yaxing Li Xue Li Ping Zhang Lianxue |
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| Institution: | Li Ran,Zang Pu,Gao Yugang,Ma Lin,Wang Yaxing,Li Xue,Li Ping,Zhang Lianxue(Collage of Traditional Chinese Medicine,Jilin Agricultural University,Changchun 130118) |
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| Abstract: | Ginseng hairy roots were induced by leaf disc method,and recombinant plant expression vector(pBI121/E0)of BVDV geneE0 of deer was transformed into ginseng hairy roots byAgobacterium rhizgenses with Ri-plasmid.E0 in ginseng hairy roots was detected by PCR,RT-PCR methods.The results indicated that E0 transgenic ginseng hairy roots were obtained.E0 was transferred into Ginseng hairy roots,and expressed in ginseng hairy roots. |
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| Keywords: | BVDV E0 ginseng hairy roots RT-PCR |
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