| 草茎点霉粗毒素的产生条件及活性测定 |
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| 引用本文： | 纪明山,刘聃,谷祖敏,吴德财,王毅婧,刘畅.草茎点霉粗毒素的产生条件及活性测定[J].中国农学通报,2011,27(10):153-156. |
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| 作者姓名： | 纪明山 刘聃 谷祖敏 吴德财 王毅婧 刘畅 |
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| 作者单位： | 沈阳农业大学植物保护学院,沈阳,110866 |
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| 摘 要： | 为了寻求能够充分发挥草茎点霉粗毒素活性的最佳培养条件，笔者利用有机溶剂萃取草茎点霉发酵液，获得粗毒素，采用针刺法、种子萌发法确定草茎点霉毒素合适的提取条件及对鸭跖草的致病性。结果表明：草茎点霉胞外毒素活性较高，3种有机溶剂中乙酸乙酯萃取效果最好，产毒的最佳条件为：培养温度32℃、培养时间14天、培养方式为150 r/min震荡培养。粗毒素浓度在1000 μg/mL时种子萌发抑制率为26.09%，病斑面积达到6.85 mm2。萃取毒素采用有机溶剂的种类以及培养温度、培养时间、培养方式对草茎点霉粗毒素的活性影响很大。
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| 关 键 词： | 方案 方案 |
| 收稿时间： | 2010/11/11 0:00:00 |
| 修稿时间： | 2011/1/15 0:00:00 |
Activity and Producing Conditions of Phoma herbarum Toxin |
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| Ji Mingshan,Liu Dan,Gu Zumin,Wu Decai,Wang Yijing,Liu Chang.Activity and Producing Conditions of Phoma herbarum Toxin[J].Chinese Agricultural Science Bulletin,2011,27(10):153-156. |
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| Authors: | Ji Mingshan Liu Dan Gu Zumin Wu Decai Wang Yijing Liu Chang |
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| Institution: | Ji Mingshan,Liu Dan,Gu Zumin,Wu Decai,Wang Yijing,Liu Chang(College of Plant Protection,Shenyang Agricultural University,Shenyang 110866) |
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| Abstract: | In order to determine the optimum culture conditions ofPhoma herbarum for the toxin production,the author acquired the crude toxin of thePhoma herbarum by the way of organic solvent extraction,and the extraction condition was optimized by the seed germination and seedling stage stabbing inoculation.The results showed that this crude toxin showed high inhibitory activity,and the best extract organic solvent was ethylacetate.Besides the best conditions of toxin production of this strain were as follows:the fe... |
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| Keywords: | Phoma herbarum toxin toxin-producing activity |
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