| 酶法制备鲽鱼鱼皮胶原蛋白肽及其清除超氧阴离子自由基的研究 |
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| 引用本文： | 王群,郑海涛,葛尧,何计国.酶法制备鲽鱼鱼皮胶原蛋白肽及其清除超氧阴离子自由基的研究[J].中国农学通报,2011,27(14):87-93. |
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| 作者姓名： | 王群 郑海涛 葛尧 何计国 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 采用碱性蛋白酶酶解鲽鱼鱼皮胶原蛋白制备胶原蛋白肽并对其清除超氧阴离子自由基的能力进行研究。通过单因素试验和响应面法分析不同酶解条件对超氧阴离子自由基清除率的影响，优化得到最佳酶解工艺条件：pH 9.5，加酶量1581 U/g，底物浓度10 mg/mL，酶解时间为6 h，酶解温度60℃，最高清除率为75.51%，半数清除浓度(IC50)为7.98 mg/mL。
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| 关 键 词： | 鲽鱼鱼皮 胶原蛋白肽 超氧阴离子自由基 响应面法 |
| 收稿时间： | 3/2/2011 12:00:00 AM |
| 修稿时间： | 2011/4/11 0:00:00 |
Study on the Enzymatic Preparation and Superoxide Anion Radical Scavenging Activity of Collagen Peptide from Plaice Skin |
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| Wang Qun,Zheng Haitao,Ge Yao,He Jiguo.Study on the Enzymatic Preparation and Superoxide Anion Radical Scavenging Activity of Collagen Peptide from Plaice Skin[J].Chinese Agricultural Science Bulletin,2011,27(14):87-93. |
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| Authors: | Wang Qun Zheng Haitao Ge Yao He Jiguo |
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| Institution: | Wang Qun,Zheng Haitao,Ge Yao,He Jiguo ( College of Food Science & Nutritional Engineering,China Agricultural University,Beijing 100083) |
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| Abstract: | Alcalase was used to hydrolyze plaice skin for preparing collagen peptide, and the superoxide anion radical scavenging activity of this collagen peptide was studied. Effect of the different enzymatic hydrolysis conditions on superoxide anion radical scavenging activity were analyzed by single factor experiment and response surface method. The optimal hydrolytic conditions of plaice skin collagen using Alcalase with high scavenging rate were pH=9.5, enzyme dosage 1581 U/g, concentration of substrate 10 mg/mL... |
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| Keywords: | plaice skin collagen peptide superoxide anion radical response surface method |
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