中草药复方制剂对猪口蹄疫免疫过程中NK细胞的影响

将70头30日龄仔猪随机分成7组，每组10头，在口蹄疫免疫过程中按1%添加剂量使用中草药复方制剂，Ⅰ、Ⅱ、Ⅲ组分别在免疫前5天、3天、当天开始连续饲喂3天，Ⅳ、Ⅴ、Ⅵ组分别在免疫前5天、3天、当天饲喂1天。对照组（Ⅶ组）不喂药。分别于免疫前一天和免疫后14d、21d、28d和二免后28d，采血检测NK细胞数量。结果表明：投药组NK细胞数量均高于对照组，Ⅰ、Ⅱ、Ⅲ组NK细胞数量高于Ⅳ、Ⅴ、Ⅵ组，Ⅱ组NK细胞数量最高，其中Ⅰ、Ⅱ、Ⅲ组与对照组差异显著（P<0.05），Ⅳ、Ⅴ、Ⅵ组与对照组差异不显著（P>0.05）。

Effect of Chinese herb complex on NK cells in the foot and mouth disease immune process of pig

70 piglets which were in the same age were randomly allotted into 7 groups. Pigs in group Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ were fed a basal diet supplemented with 1% Chinese herb complex respectively; while animals in group Ⅶ were fed the basal diet only as a control in the foot and mouth disease immune process. Pigs in group Ⅰ, Ⅱ, Ⅲ were immune before 5 days, 3 days, the same day continuous feeding 3 days; group Ⅳ, Ⅴ, Ⅵ were immune before 5 days, 3 days, the same day of feeding one day. These pigs were collected Blood samples and detected NK cells on the day before immunization, 14 days, 21 days, 28 days after immunization and the 28 days after second immunization. The results showed that NK cells in dosage group were higher than control group. NK cells of group Ⅰ, Ⅱ, Ⅲ were higher than group Ⅳ, Ⅴ, Ⅵ NK cells in group Ⅱ were the highest. There was a statistical difference of pig’s NK cells in group Ⅰ, Ⅱ, Ⅲ and control group （P0.05）.