套袋和抑制剂处理对桃果皮中葡萄糖-6-磷酸脱氢酶以及花色苷的影响

研究套袋处理和细胞G6PDH活性抑制剂-脱氢异雄酮（DHEA）涂抹处理，探讨桃果实着色过程中G6PDH、PAL活性与花色苷的关系。以‘早玉’桃（Prunus persica L. Batsch cv. ‘zaoyu’）为试材，研究了套袋对果皮花色苷、G6PDH和PAL活性、G6PDH亚细胞分布的影响，也试用DHEA处理，观察果皮颜色变化，并测定果皮中G6PDH活性和花色苷含量。自然生长的果实中，花色苷与G6PDH、PAL活性变化趋势相同；套袋果实摘除套袋后，G6PDH和PAL活性、花色苷都迅速增加，而未摘掉套袋的果实中，G6PDH、PAL活性和花色苷的含量基本没有变化；果实G6PDH主要存在于细胞质和质体（转色前为叶绿体，而转色期为杂色体）中，套袋使得叶绿体狭长，而且发生叶绿体裂殖；果皮质体中胶体金颗粒数量与G6PDH活性变化一致；脱氢异雄酮（DHEA）处理降低了G6PDH活性，花色苷含量也明显减少。套袋处理果实中G6PDH、PAL活性和花色苷有一定的相关关系，抑制G6PDH的活性能够抑制花色苷的合成。进一步表明G6PDH在果实花色苷合成中具有关键作用。

Influence of Fruit Bagging and DHEA on Glucose-6-phosphate Dehydrogenase and Anthocyanin of Peach Fruit

This experiment was carried out to study the relationships among glucose-6-phosphate dehydrogenase (G6PDH),phenylalanine ammonia-lyase (PAL) activity and the concentrations of anthocyanin,by using fruit with bagging and DHEA treatment.Fruits from nine-year-older peach trees (Prunus persica L.Batsch cv ' zaoyu ') were used as materials in this study.Fruits were bagged at the pit-hardening stage (with removed and without),non-bagged were used as control.And the concentration of anthocyanin,the activities of P...