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| 犬淋巴细胞转化增殖试验最佳条件的确定 | |
| 引用本文： | 孔庆波,刘剑郁,常建军,陈德坤.犬淋巴细胞转化增殖试验最佳条件的确定[J].中国农学通报,2007,23(7):46-46. |
| 作者姓名： | 孔庆波 刘剑郁 常建军 陈德坤 |
| 作者单位： | 1. 中国刑事警察学院警犬技术系,辽宁沈阳,110034;西北农林科技大学动物科技学院,陕西杨凌,712100 2. 中国刑事警察学院警犬技术系,辽宁沈阳,110034 3. 青海大学农牧学院,青海西宁,810003 4. 西北农林科技大学动物科技学院,陕西杨凌,712100 |
| 基金项目： | 公安部科技局基金项目（20045829501）. |
| 摘 要： | 本试验选择杂种牧羊犬作为实验犬，用MTT法检探讨了不同浓度犊牛血清（FCS）、淋巴细胞、植物血凝素P（PHA-P）和刀豆蛋白A（ConA）对犬淋巴细胞增殖的影响。确定了犊牛血清含量为10%，淋巴细胞浓度为5×106个/mL，用丝裂原PHA-P 刺激的最佳浓度为12.5ug/mL时，MTT法测定能够刺激犬淋巴细胞转化增殖，但t检验分析表明其促进淋巴细胞增殖的效果并不显著；当其它条件不变，所用的丝裂原为Con A时，它在浓度为0.5～2.5μg/mL范围内均可以显著刺激T细胞的增殖（P<0.05），且在浓度为2.5μg/mL时刺激效果最佳 |
| 关 键 词： | 基因组文库 基因组文库 西农萨能奶山羊 |
| 修稿时间： | 2007-04-092007-04-28 |
The Test Condition on the Test of Canine Lymphocytes Proliferation |
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| Kong Qingbo,Liu Jianyu,Chang Jianjun,Chen Dekun.The Test Condition on the Test of Canine Lymphocytes Proliferation[J].Chinese Agricultural Science Bulletin,2007,23(7):46-46. | |
| Authors: | Kong Qingbo Liu Jianyu Chang Jianjun Chen Dekun |
| Institution: | (1Dept. of Police Dog Technology, Criminal Police College of China, Shenyang 110034; 2College of Animal Science and Veterinary Medicine, Northwest A＆F University, Yangling 712100; 3College of Agricultral and Animal Husbandry,Qinghai University, Xining 81 |
| Abstract: | In this present test, a range of hybrid shepherd dogs were selected firstly. Afterwards, the effects of FCS，PBMC，PHA-P of different concentration on promote lymphocytes proliferation were tested by MTT test. The result of experiment demonstrate that the best levels combination of canine lymphocyte，calf blood serum were 5×106 unit/ml， 10% respectively. In the lymphocytic transfer test, PHA-P at dosage of 12.5μg/ ml performed facilitation effects for canine lymphocytic proliferation (p>0.05). ConA at 0.5~2.5μg/ mL markedly proliferated T lymphocytes (p<0.05), with the best effect at 2.5μg/ ml. |
| Keywords: | canine lymphocytes MTT test proliferation |
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