甜菜全基因组SSR引物的筛选与评价

旨在筛选出适合于甜菜分子生物学实验的SSR核心引物,为后续构建甜菜指纹图谱及遗传多样性分析奠定重要基础。本文利用4种不同的甜菜种质资源和4个不同的甜菜品种,对基于甜菜全基因组编码区序列设计的247对SSR引物进行了初步筛选。结果表明,247对引物中绝大多数引物没有多态性或者多态性不好,只有27对引物具有较高的多态性,这27对引物总共扩增出103条带,其中多态性条带95条,多态性比率为90.43%。这些引物多态性信息量(PIC)范围为0.549~0.983,平均每一对引物的PIC值为0.841。试验结果表明虽然经过了电子PCR的筛选,想要找到合适的甜菜SSR引物依然比较困难,说明甜菜的遗传基础比较狭窄。这27对SSR引物适用于甜菜分子标记,为甜菜品种标准化鉴定体系的大规模构建和应用奠定了重要基础。

The Whole Genome SSR Primers of Sugar Beet: Screening and Evaluation

The aim is to screen out SSR core primers suitable for sugar beet molecular biology experiments, and lay a foundation for subsequent construction of sugar beet fingerprint maps and genetic diversity analysis. In this paper, four different sugar beet germplasm resources and four different sugar beet varieties were used for the preliminary screening of 247 pairs of SSR primers designed based on the sequence of the whole genome coding region of sugar beet. The results showed that the majority of the 247 primer pairs had no polymorphism or poor polymorphism, while only 27 primer pairs had high polymorphism. These 27 primer pairs amplified a total of 103 bands, including 95 polymorphic bands, with a polymorphism ratio of 90.43%. The polymorphism information content (PIC) of these primers ranged from 0.549 to 0.983, with an average PIC value of 0.841 for each primer pair. The results of the experiment showed that despite the screening by e-PCR, it was still difficult to find suitable SSR primers for sugar beet, indicating that the genetic base of sugar beet was relatively narrow. These 27 pairs of SSR primers are suitable for sugar beet molecular marking, and could be a foundation for large-scale construction and application of the standardized identification system for sugar beet varieties.