| Phenotype Variations in Plant Regeneration of Upland Cotton (Gossypium hirsutum L.) |
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| 作者姓名： | 迟吉娜 马峙英 韩改英 李喜焕 王彦霞 王省芬 |
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| 作者单位： | 河北农业大学/河北省作物种质资源重点实验室,保定,071001 |
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| 基金项目： | 河北省科技攻关项目(03220137D);河北省博士资金项目(04547001D-2). |
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| 摘 要： | 以陆地棉冀无2031无菌苗下胚轴为外植体，在MS中附加激素0.1mg/L IAA+0.1mg/L 2,4-D+0.1mg/L KT的培养基上诱导出愈伤组织。选择疏松愈伤组织继代到附加0.1mg/L ZT的培养基中，经2~3个月的继代培养诱导出颗粒状疏松胚性愈伤组织。胚性愈伤组织在无激素、每升加2g谷氨酰胺和0.5g天门冬酰胺的胚性愈伤增殖萌发培养基上培养2个月左右，可陆续形成不同时期的体胚苗。对冀无2031不同时期的体细胞萌发后的体胚苗进行观察，发现了真叶畸形苗、子叶节生根胚、胚轴侧生根胚、腋芽丛生苗和以腋
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| 关 键 词： | 陆地棉 体细胞 植株再生 变异 |
Phenotype Variations in Plant Regeneration of Upland Cotton (Gossypium hirsutum L.) |
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| Chi Jin,Ma Zhiying,Han Gaiying,Li Xihuan,Wang Yanxi,Wang Xingfen.Phenotype Variations in Plant Regeneration of Upland Cotton (Gossypium hirsutum L.)[J].Chinese Agricultural Science Bulletin,2005,21(4):59-59. |
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| Authors: | Chi Jin Ma Zhiying Han Gaiying Li Xihuan Wang Yanxi Wang Xingfen |
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| Institution: | (Key laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding 071001) |
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| Abstract: | A variety of upland cotton, Jiwu2031, was studied on somatic embryogenesis and plant regeneration. Callus was initiated from hypocotyls explants on a callus initiation medium, modified MS with 0.1mg/L IAA+0.1mg/L2，4-D+0.1mg/L KT. Friable callus was period |
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| Keywords: | Upland cotton Somatic embryogenesis Plant regeneration Phenotype |
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