| 荷叶离褶伞菌株的ITS鉴定及生物学特性研究 |
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| 引用本文： | 王守现,刘宇,许峰,赵爽,耿小丽,王兰青,孟莉莉.荷叶离褶伞菌株的ITS鉴定及生物学特性研究[J].中国农学通报,2012,28(28):148-152. |
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| 作者姓名： | 王守现 刘宇 许峰 赵爽 耿小丽 王兰青 孟莉莉 |
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| 作者单位： | 北京市农林科学院植物保护环境保护研究所,北京100097 |
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| 基金项目： | 北京市农林科学院青年基金项目“野生黄伞菌株的评价及高效栽培技术研究”（QN201109）; 北京市科技新星项目A类（2009A29） |
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| 摘 要： | 为更好地开发利用荷叶离褶伞，本研究以1株离褶伞属野生菌为研究对象，通过分子生物学鉴定及测定菌丝生长速度、菌丝干重和生长势等指标，研究其生物学特性。结果表明该菌经鉴定为荷叶离褶伞，其菌丝生长的最适碳源为麦芽糖，最适氮源为大豆蛋白胨，最适碳氮比为30/1，最适生长因子为维生素B6，最适生长温度为20℃，最适pH值为6.0。本研究结果可为荷叶离褶伞生产栽培提供一定的理论依据。
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| 关 键 词： | 抗早花 抗早花 |
| 收稿时间： | 5/7/2012 12:00:00 AM |
| 修稿时间： | 2012/7/22 0:00:00 |
Study on Identification and Biological Characteristics of Wild Lyophyllum decastes Strain |
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| Wang Shouxian,Liu Yu,Xu Feng,Zhao Shuang,Geng Xiaoli,Wang Lanqing,Meng Lili.Study on Identification and Biological Characteristics of Wild Lyophyllum decastes Strain[J].Chinese Agricultural Science Bulletin,2012,28(28):148-152. |
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| Authors: | Wang Shouxian Liu Yu Xu Feng Zhao Shuang Geng Xiaoli Wang Lanqing Meng Lili |
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| Institution: | (Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097) |
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| Abstract: | In order to make better development and use of Lyopbyllum decastes, a wild mushroom, identified by molecular biological techniques as L. decastes （Fr.：Fr.） Sing., was tested and the biological characteristics were researched by measuring mycelial growth rate, dry weight and growth vigor. The results showed that the optimal carbon source was maltose and the optimal nitrogen source was soybean peptone. The most suitable carbon nitrogen ratio was about 30/1 and the optimal growth factor was vitamin B6; the optimal temperature and pH were found to be 20℃ and 6.0, respectively. The results of this study may provide a theoretical basis for the cultivation of L. deeastes. |
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| Keywords: | Lyophyllum decastes isolation identification biological characteristic |
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