CRISPR/Cas9介导的MSTN/FGF5基因编辑陕北白绒山羊粪便菌群分析

为了从绒山羊粪便微生物群落变化的角度对MSTN/FGF5基因编辑陕北白羊绒山的自身健康和环境安全性进行研究，利用6种不同培养基分别对MSTN/FGF5基因编辑和野生型陕北白绒山羊粪便不同微生物进行培养、计数，并做菌群结构的显著性检验分析。结果表明，FGF5/MSTN基因编辑和野生型陕北白绒山羊F0代和F1代公羊和母羊新鲜粪样经LB琼脂、伊红美蓝琼脂、TPY琼脂、胆硫乳琼脂、肠球菌琼脂和甘露醇盐琼脂培养后微生物计数分别为，F0代：公羊6.35±0.68，5.89±0.25；5.32±0.10，5.27±0.34；6.64±0.57，6.71±0.35；5.03±0.53，5.32±0.14；4.12±0.13，4.15±0.21；5.34±0.18，5.25±0.25；母羊6.62±0.45，6.64±0.31；4.80±0.51，4.75±0.45；7.20±0.30，7.15±0.55；4.30±0.52，4.42±0.62；5.83±0.51，5.73±0.86；6.24±0.28，6.24±0.21。F1代：公羊6.92±0.12，7.08±0.04；4.02±0.64，3.99±0.51；6.32±0.15，6.25±0.30；5.21±0.28，5.17±0.20；3.66±0.50，3.88±0.67；4.06±0.50，3.97±0.46；母羊7.12±0.24，6.96±0.19；4.15±0.19，4.25±0.24；6.45±0.30，6.56±0.45；4.97±0.42，4.87±0.38；4.04±0.96，3.96±0.57；4.10±0.28，3.85±0.37。F0和F1代MSTN/FGF5基因编辑陕北白绒山羊与野生型陕北白绒山羊粪便中菌群结构之间不存在显著性差异(P>0.05)。本研究为FGF5/MSTN基因编辑陕北白绒山羊的安全性评价研究提供了试验数据和研究基础。

Fecal Microbial Flora Analysis in MSTN/FGF5 Gene-modified Shaanbei White Cashmere Goat Mediated by CRISPR/Cas9

In order to assess the MSTN/FGF5 gene modified Shaanbei cashmere goats on the health of animal themselves and on the safety of environment from the aspect of the changes in microbe population of goat faeces, six different kinds of media were used to count fecal bacteria of the MSTN/FGF5 gene modified and wild-type goats, respectively, microbial flora and significance test analysis was also conducted. Results of microbial flora of feces which were cultured on the different media including Luria-Bertani, Bile Esculin Azide, DHL agar, Mannitol salt selective, Eosin methylene blue agar and TPY plates were as follows: F0, male, 6.35±0.68, 5.89±0.25; 5.32±0.10, 5.27±0.34; 6.64±0.57, 6.71±0.35; 5.03±0.53, 5.32±0.14; 4.12±0.13, 4.15±0.21; 5.34±0.18, 5.25±0.25; female 6.62±0.45, 6.64±0.31; 4.80±0.51, 4.75±0.45; 7.20±0.30, 7.15±0.55; 4.30±0.52, 4.42±0.62; 5.83±0.51, 5.73±0.86; 6.24±0.28, 6.24±0.21。F1: male 6.92±0.12, 7.08±0.04; 4.02±0.64, 3.99±0.51; 6.32±0.15, 6.25±0.30; 5.21±0.28, 5.17±0.20; 3.66±0.50, 3.88±0.67; 4.06±0.50, 3.97±0.46; female 7.12±0.24, 6.96±0.19; 4.15±0.19, 4.25±0.24; 6.45±0.30, 6.56±0.45; 4.97±0.42, 4.87±0.38; 4.04±0.96, 3.96±0.57; 4.10±0.28, 3.85±0.37. Results indicated that in comparison with the control goats, microbe population of various species in feces of MSTN/FGF5 gene modified goats remained unchanged (P>0.05). In summary, this study provided the experimental data and research basis for the safety evaluation of MSTN/FGF5 gene modified Shaanbei cashmere goat.