甜菜DAMD引物的筛选及不同凝胶系统对扩增产物多态性的影响

为了筛选适宜于甜菜品种纯度鉴定的DAMD引物,笔者利用12个不同的甜菜品种分别对26条DAMD引物进行扩增,并分别采用6%聚丙烯酰胺凝胶电泳和2%的琼脂糖凝胶电泳对扩增产物进行检测。结果表明,从26条DAMD引物中筛选出16条扩增清晰的引物,这16条引物共扩增出139条带,其中多态性条带为122条,多态性百分比为87.7%,这16条引物可以作为甜菜品种纯度和真实性鉴定的核心引物,同时发现聚丙烯酰胺凝胶电泳与琼脂糖凝胶电泳的检测结果差异不大,而琼脂糖凝胶具有制作方便、检测简单以及不使用任何有毒药剂等优点,推荐甜菜DAMD扩增产物的检测使用琼脂糖凝胶电泳。

Screening of Beet DAMD Primers and the Effects of Different Gel Systems on Polymorphism of Amplified Products

To select suitable for sugar beet varieties purity identification of DAMD primers, respectively using 12 different sugar beet varieties of 26 DAMD primers for amplification, and 6% respectively using polyacrylamide gel electrophoresis and 2% agarose gel electrophoresis, respectively, to test the amplification products.Results from 26 DAMD article 16 primers screened in clear primer amplification, this article 16 primers amplification out 139 bands, including polymorphism bands for 122, the percentage of polymorphic (87.7%), the 16 primers can be used as a sugar beet varieties purity and authenticity identification of core primers, also found that the polyacrylamide gel electrophoresis and agarose gel electrophoresis results difference is not big, but the agarose gel is easy to make, test the advantages of simple and does not apply to any potions, recommend beet DAMD amplification product detection using agarose gel electrophoresis.