| 牛体细胞核移植技术体系的优化 |
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| 引用本文： | 潘兴乾,詹同彤,杨健,倪和民,郭勇.牛体细胞核移植技术体系的优化[J].中国农学通报,2017,33(5):95-100. |
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| 作者姓名： | 潘兴乾 詹同彤 杨健 倪和民 郭勇 |
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| 作者单位： | 北京农学院,北京农学院动物育种与辅助生殖实验室,北京农学院动物育种与辅助生殖实验室,北京农学院动物育种与辅助生殖实验室,北京农学院动物育种与辅助生殖实验室 |
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| 基金项目： | 2015 年国家自然科学基金项目“MUC-1 协调GlyCAM调控人工诱导发情绵羊子宫容受态的研究”(31470118)；奶牛产业技术体系北京市 创新团队。 |
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| 摘 要： | 为了提升卵母细胞成熟质量和核移植胚胎的体外发育潜能,在卵母细胞成熟液和胚胎培养液中分别添加不同浓度IGF及胎牛血清FBS,并探讨了二者对牛卵母细胞成熟培养、核移植胚胎发育的影响以及相关性能的变化。结果表明:分别在成熟液中添加40 ng/mL IGF、培养液中添加20%FBS后,成熟率、卵裂率、囊胚率均有显著提高。本研究为今后牛体细胞核移植技术体系的优化和完善打下了坚实的理论基础。
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| 关 键 词： | 类胰岛素生长因子 胎牛血清 体外成熟 体细胞核移植 胚胎培养 |
| 收稿时间： | 2016/6/24 0:00:00 |
| 修稿时间： | 2017/1/6 0:00:00 |
Optimization of Bovine Somatic Cell Nuclear Transfer Technology System |
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| Abstract: | To improve the quality of oocyte maturation and developmental competence of nuclear transfer embryos, different concentrations of IGF and FBS were added into oocyte mature medium and embryo nutrient medium, respectively. Then maturation rate, cleavage rate, blastocyst rate were tested to explore the effect of IGF and FBS on bovine oocyte maturation culture and nuclear transfer embryo development. The results showed that adding 40 ng/mL IGF in mature medium and 20% FBS in nutrient medium, maturation rate, cleavage rate, blastocyst rate were significantly increased. The results would lay a foundation for optimizing bovine somatic cell nuclear transfer technology in the future. |
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| Keywords: | FBS in vitro maturation somatic cell nuclear transfer embryo culture |
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