导入TaNHX2基因提高中林美荷杨耐盐性的研究

中林美荷杨具有生长速度快、扦插成活率高及木材材质好等优点，因而倍受欢迎，是西部大开发重点推广的绿化造林树种。为提高该树种的耐盐性，充分适应西北广大地区干旱盐碱地，将小麦耐盐基因TaNHX2导入中林美荷杨基因组中，以提高其的耐盐性。采用叶片和叶柄作为外植体，通过根癌农杆菌(Agrobacterium tumefaciens)介导法，将TaNHX2导入中林美荷杨基因组中，对所获得的杨树转基因植株进行PCR和Southern blotting分析，结果表明TaNHX2基因多以单拷贝整合到受体基因组中。转基因幼苗经过不同浓度的NaCl胁迫处理，表现出一定的耐盐性。中林美荷杨耐盐株系的获得为该树种绿化造林的推广和丰产提供了保障。

Study on Introduction of TaNHX2 Gene to Enhance Salt Tolerance of Populus sp.

Zhonglinmeihe is an elite variety of Populus sp. which is fast-growing, and has high yield and high quality of timber. It is important for afforestation programs in the development of China’s western regions. To enhance salt tolerance and widen adaptability of Populus sp. in northwest China, TaNHX2 gene was introduced into the variety through Agrobacterium-mediated transformation with explants of leaf and petiole. Based on PCR and Southern blotting analyses, most transgenic plants showed one copy of insertion of the target gene. Under various concentrations of NaCl treatment, transgenic poplar plants exhibited obvious resistance to salt stress. All in all, transgenic poplar plants with salt tolerance could facilitate afforestation and achieve high yield in northwest China.