| 黑龙江省野生黑木耳菌株遗传多样性分析 |
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| 引用本文： | 陶朋飞,许修宏,刘华晶.黑龙江省野生黑木耳菌株遗传多样性分析[J].中国农学通报,2011,27(22):182-186. |
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| 作者姓名： | 陶朋飞 许修宏 刘华晶 |
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| 作者单位： | 东北农业大学资源与环境学院,哈尔滨,150030 |
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| 基金项目： | 国家“十一五”科技支撑项目(2008BADA1B01); 东北农业大学创新团队发展计划基金资助(CXT003-2-1) |
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| 摘 要： | 利用SRAP标记对黑龙江地区采集的30株野生黑木耳菌株进行遗传多样性分析，利用8对扩增条带清晰、多态性丰富、稳定性较好的引物对30个供试菌株进行SRAP扩增，共得到157条重复性良好的DNA条带，其中多态性条带122条，占77.71%；使用UPGMA法构建进化树，30个菌株被分为6组。结果表明，黑龙江省野生黑木耳菌株间存在着丰富的遗传多样性。本研究为将来杂交育种亲本的选择提供很好的理论依据。
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| 关 键 词： | “3S”技术 “3S”技术 |
| 收稿时间： | 3/2/2011 12:00:00 AM |
| 修稿时间： | 2011/3/22 0:00:00 |
Genetic Diversity Analysis of Wild Auricularia auricul -Jude from Heilongjiang Province |
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| Tao Pengfei,Xu Xiuhong,Liu Huajing.Genetic Diversity Analysis of Wild Auricularia auricul -Jude from Heilongjiang Province[J].Chinese Agricultural Science Bulletin,2011,27(22):182-186. |
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| Authors: | Tao Pengfei Xu Xiuhong Liu Huajing |
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| Institution: | Tao Pengfei,Xu Xiuhong,Liu Huajing(School of Resources and Environment,Northeast Agricultural University,Harbin 150030) |
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| Abstract: | 30 wild Auricularia auricula strains were collected from Heilongjiang Province and analyzed by using sequence-related amplified polymorphism(SRAP) markers.8 SRAP primer combinations amplified 157 fragments of which 122(77.71%) were polymorphic.Phylogenetic tree was constructed by using the Unweighted Pair-group Method with Arithmetic Averages(UPGMA) method.30 strains distributed into six groups.The clustering analysis showed that there was a high level of genetic diversity among A.auricula.This study would ... |
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| Keywords: | Auricularia auricula SRAP markers genetic diversity cluster analysis |
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