| 山羊心脏型脂肪酸结合蛋白cDNA序列克隆及生物信息学分析 |
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| 引用本文： | 王兰萍,耿荣庆,冀德君,常洪.山羊心脏型脂肪酸结合蛋白cDNA序列克隆及生物信息学分析[J].中国农学通报,2011,27(23):17-20. |
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| 作者姓名： | 王兰萍 耿荣庆 冀德君 常洪 |
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| 作者单位： | 1. 盐城师范学院生命科学与技术学院,江苏盐城,224051
2. 扬州大学动物科学与技术学院,江苏扬州,225009 |
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| 基金项目： | 江苏省高校自然科学基础研究项目“黄淮山羊H-FABP基因多态性与IMF含量的相关研究”(09KJD230001) |
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| 摘 要： | 本文通过RT-PCR技术克隆山羊心脏型脂肪酸结合蛋白的cDNA 全序列，并对cDNA序列的氨基酸同源性、理化性质、二级结构特征进行了预测和分析。结果显示，山羊H-FABP基因cDNA全长402 bp，推测的氨基酸序列与绵头同源性为100%；H-FABP蛋白属疏水性蛋白，呈为弱酸性；二级结构以β折叠为主；不含跨膜结构域，具有潜在的信号肽位点、多个其它信号位点以及细胞脂肪酸结合信号位点，从而为揭示H-FABP蛋白空间结构和生物学功能研究提供理论基础。
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| 关 键 词： | 葛氏鲈塘鳢 葛氏鲈塘鳢 染色体组型 染色体数目 |
| 收稿时间： | 2011/1/11 0:00:00 |
| 修稿时间： | 2011/2/10 0:00:00 |
cDNA Clonging of Heart Fatty Acid Binding Protein Gene from Goat and its Bioinformatic Analysis |
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| Wang Lanping,Geng Rongqing,Ji Dejun,Chang Hong.cDNA Clonging of Heart Fatty Acid Binding Protein Gene from Goat and its Bioinformatic Analysis[J].Chinese Agricultural Science Bulletin,2011,27(23):17-20. |
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| Authors: | Wang Lanping Geng Rongqing Ji Dejun Chang Hong |
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| Institution: | Wang Lanping 1,Geng Rongqing 1,Ji Dejun 2,Chang Hong 2 (1 College of life science and technology,Yancheng teachers university,Yancheng Jiangsu 224051,2 College of animal science and technology,Yangzhou university,Yangzhou Jiangsu 225009) |
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| Abstract: | Full length cDNA sequence of heart fatty acid binding protein from goat was cloned by RT-PCR technology.The analysis and prediction on the amino similarity,physicochemical properties and secondary structure character were conducted from acquired sequences.The results showed that goat H-FABP gene cDNA was 402 bp in length and its homology rates of predicted amino acid sequence was 100% to sheep.H-FABP belong to hydrophilic and faintly acid protein.The secondary structure was mainly composed of β sheet.It had no transmembranous regions but possessed potential signal peptide,several other signal sites and cell fatty acid binding site.These results would provide theoretical base for revealing the protein space structure and biological function. |
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| Keywords: | goat heart fatty acid binding protein cloning sequence analysis |
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