桑黄菌丝体中多糖提取工艺优化及其体外抗氧化活性分析

为了研究桑黄菌丝体中桑黄多糖的提取工艺及其体外抗氧化活性,利用热水浸提法,在单因素实验结果的基础上,采用多因素正交试验对桑黄菌丝体中桑黄多糖的提取工艺进行优化,并通过检测桑黄多糖清除ABTS+.和DPPH.自由基的能力来初步评价其体外抗氧化活性。结果表明：桑黄多糖最佳提取工艺为提取时间2.5 h,提取温度60 ℃,提取次数3次,液料比为14倍,在该最佳提取条件下桑黄多糖得率为4.97%；体外抗氧化活性实验结果表明,桑黄多糖的ABTS+.和DPPH.自由基清除能力有良好的剂量－效应关系,对ABTS+.和DPPH.的最高清除率分别为73.54%和88.83%。表明优化的桑黄液体发酵菌丝体中桑黄多糖提取工艺合理、可行,桑黄多糖有较强的体外抗氧化活性,可用于功能性食品和药剂的开发利用。

Optimization of Extraction Process of Polysaccharide of Phellinus igniarius Mycelium and Analysis of Its Antioxidant Activity in vitro

To study extraction process of polysaccharide from Phellinus igniarius mycelium and its antioxidant activity in vitro, according to hot water extraction and based on the single factor experiments, the extraction condition of polysaccharide from Phellinus igniarius was optimized using orthogonal test and its antioxidant activity in vitro was primarily evaluated by ABTS+. and DPPH. free radical scavenging ability. The results showed that the optimal extraction conditions were determined as follows: extraction time 2.5 hours, extraction temperature 60 ℃, number of extraction 3 times and liquid-to-solid ratio of 14 times. Under these conditions, the average extraction yield was 4.97%. The result of antioxidant activity in vitro experiments showed that the highest scavenging ability of polysaccharides against ABTS+. and DPPH. was 73.54% and 88.83% respectively. The optimization extraction process of polysaccharide from Phellinus igniarius Mycelium was reasonable and feasible, and it had stronger antioxidant activity and had a potential exploited value in functional foods and medicines.