暗黑赤眼蜂低温贮存技术研究

旨在对暗黑赤眼蜂低温贮存技术进行研究，以期为规模化繁殖的暗黑赤眼蜂低温贮存提供依据。利用清水、0.5%盐水、1.0%盐水、1.5%盐水和直接冷藏等方式处理被暗黑赤眼蜂寄生的麦蛾卵，研究其低温贮藏技术。结果表明：与对照相比较，0.5%、1.0%和1.5%盐水处理后能够显著提高暗黑赤眼蜂的羽化率、降低其羽化畸形率；45 天以内，各浓度盐水处理的暗黑赤眼蜂单雌产卵量与对照之间差异不显著，表明在此时间内利用盐水处理可显著保持暗黑赤眼蜂的单雌产卵量；冷藏90 天后，暗黑赤眼蜂雌雄比例开始增大，75 天后，暗黑赤眼蜂的雌雄比例失调较为严重。研究表明：冷藏时间的增加不利于暗黑赤眼蜂各项指标的发育，但0.5%、1.0%和1.5%盐水处理后能在一定程度上减少负面影响，是羽化率、羽化畸形率、雌虫寿命、单雌产卵量和雌雄比例等指标的有利因素。

Trichogramma pintoi Voegele: Study on Cryopreservation Techniques

The paper aims to study cryopreservation techniques of Trichogramma pintoi Voegele, and to provide references for large-scale breeding. We utilized different treatments such as water, 0.5%, 1.0%, 1.5% of saline water and cryopreservation directly, studied cryopreservation techniques of Trichogramma pintoi Voegele. The results showed that: compared with contrast, the treatments with 0.5%, 1.0%, 1.5% of saline water could improve eclosion rate and reduce eclosion aberration rate; the fecundity per female treated by each concentration of saline water had no significant difference compared with the contrast within 45 days, indicating that Trichogramma pintoi Voegele treated by each concentration of saline water could maintain fecundity per female within 45 days; the female-male rate increased after cryopreservation for 90 days, and female-male rate became imbalanced after cryopreservation for 75 days. The research also showed that: the cryopreservation time increase was against the development of richogramma pintoi Voegele, whereas the treatments with 0.5%, 1.0%, and 1.5% saline water could reduce negative effects, which were favorable factors on eclosion rate, eclosion aberration rate, female life span, fecundity per female and female-male rate.