茶多酚高通量检测技术研究进展

茶多酚是茶叶的主要活性成分,具有抗氧化、抗肿瘤、抗衰老、降血糖、防辐射等多种生物学活性。然而,茶多酚的不稳定性极大限制其进一步开发应用。茶多酚易受光线、温度、pH等因素影响而发生氧化、聚合和缩合,从而使其活性改变。因此,茶多酚组分的高通量快速检测在生物学及临床上具有重要意义。高效液相色谱法、毛细管电泳法、近红外光谱法、核磁共振等均是茶多酚组分高通量检测的主要方法,但这些方法各有优劣。对茶多酚高通量快速检测技术及其相关应用进行了归纳和总结,并对其优势及存在的问题进行分析,以期为茶多酚组分的高通量检测提供参考。

High Throughput Test of Tea Polyphenols: A Review

Tea polyphenols are the main active ingredient of tea. Various studies have shown that tea polyphenols confer great beneficial effects to the health of consumers including anti-oxidation, anti-tumor, anti-aging, hypoglycemic, radiation protection and other biological activity. However, one of the greatest challenges in developing tea polyphenols is their instability. Tea polyphenols are susceptible to oxidation, polymerization and condensation due to factors such as light, temperature, pH and other factors, so that their biological activity changes. Therefore, high throughput quantitative and rapid analysis of polyphenols in tea have great biological and clinical significance. High performance liquid chromatography, capillary electrophoresis, near infrared spectroscopy and nuclear magnetic resonance are the main methods for high throughput quantitative analysis of tea polyphenols. These methods have their own advantages and disadvantages. In the present paper, it reviewed the analysis methods and applications of high throughput quantitative of tea polyphenols. Additionally, it analyzed the problems and advantages of these methods. It hoped to provide a reference for the high throughput detection of tea polyphenols.