谷子花药颜色基因Siac1的精细定位

为从分子水平上揭示谷子花药颜色性状的遗传基础，以‘E1005’为母本，‘品资39号’为父本构建F2分离群体，利用集团分离分析法(BSA)结合隐性群体分析法(RCA)，对谷子花药颜色基因Siac1进行精细定位。遗传分析表明，F2植株黄色花药与白色花药分离比例符合3:1的孟德尔分离规律，表明白色花药性状由一对隐性核基因控制。利用SSR和SV标记将Siac1初定位于第VI 染色体上标记GSA07025和GSA07037之间约282 kb的区间内。进一步利用根据亲本重测序结果新开发的InDel标记，最终将Siac1精细定位于标记MRI579和MRI583之间约94.7 kb区段内。生物信息学分析表明，该区间共有10个开放阅读框，初步推测Seita.6G227100、Seita.6G227200、Seita.6G227300和Seita.6G227900为花药颜色性状的候选基因。本研究为克隆Siac1基因、解析花药颜色发育机制奠定了一定基础。

Fine Mapping of Anther Color Gene Siac1 in Foxtail Millet

To reveal the genetic basis of anther color in foxtail millet at molecular level, an F2 segregating population derived from a crossing between‘E1005’(female parent) and‘Pinzi 39’(male parent) was used as mapping population. With bulked segregation analysis (BSA) and recessive-class analysis (RCA), the Siac1 related to anther color was finely mapped. Genetic analysis showed that the segregation ratio of yellow anther plants to white anther plants was consistent with the Mendelian segregation of 3:1 in the F2 generation, which suggested that the white anther color trait was controlled by a pair of recessive nuclear genes. The Siac1 was preliminary mapped onto an interval of about 282 kb between markers GSA07025 and GSA07037 on chromosome VI by SSR markers and SV markers. Furthermore, fine mapping of Siac1 was conducted using newly developed InDel markers based on the sequence differences between the two parents, and the results indicated that the Siac1 was narrowed down onto a genomic region of around 94.7 kb between markers MRI579 and MRI583. Bioinformatics analysis showed that there were 10 Open Reading Frames (ORFs), and Seita.6G227100, eita.6G227200, Seita.6G227300 and Seita.6G227900 could be speculated to be four candidates for Siac1. This research lays a foundation for the cloning of Siac1 and the analysis of developmental mechanism of anther color.