| 五个鲜食玉米新品种的DNA指纹图谱研究与应用 |
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| 引用本文： | 雷开荣,杨 华,吴 红,张胜恒,林 清,郝 风.五个鲜食玉米新品种的DNA指纹图谱研究与应用[J].中国农学通报,2006,22(12):387-387. |
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| 作者姓名： | 雷开荣 杨 华 吴 红 张胜恒 林 清 郝 风 |
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| 作者单位： | 重庆市农业科学研究所,重庆市农作物生物工程技术研究中心,作物遗传改良重庆市市级重点实验室,重庆,400055 |
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| 摘 要： | 利用SSR标记技术，对5个鲜食玉米新品种及其亲本的DNA指纹图谱进行了研究。从88对SSR引物中，筛选出37对在鲜食玉米中表现多态性丰富、带型清晰明显且稳定的引物，分析了鲜食玉米自交系的遗传多样性，建立了各品种的特征DNA指纹图谱及数字指纹，可以有效用于种子质量检测。
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| 关 键 词： | 鲜食玉米 DNA指纹图谱 |
| 收稿时间： | 2006-05-24 |
| 修稿时间： | 2006-05-242006-10-19 |
Study and Utilization of DNA Fingerprinting of Fresh-eating Maize by SSR Marker |
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| Lei Kairong,Yang Hu,Wu Hong,Zhang Shengheng,Lin Qing,Hao Feng.Study and Utilization of DNA Fingerprinting of Fresh-eating Maize by SSR Marker[J].Chinese Agricultural Science Bulletin,2006,22(12):387-387. |
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| Authors: | Lei Kairong Yang Hu Wu Hong Zhang Shengheng Lin Qing Hao Feng |
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| Institution: | Chongqing Institute of Agricultural Sciences, Chongqing Engineering Research Center for Crop Biotechnology, Chonhqing Key Laboratory of Crop Genetic Improvement, Chongqing 400055 |
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| Abstract: | Using SSR-PCR molecular technique, the DNA fingerprinting of 5 new fresh-eating maize hybrids and their parents were analyzed. In this study, 37 out of 88 pairs of SSR primers were selected, which gave stable and polymorphic amplification profiles in focus. Genetic diversity of fresh-eating maize inbred lines was analyzed. The characteristic DNA fingerprinting and figure fingerprinting of 5 hybrids and their parents were constructed and could be used in seed inspection and testing availably. |
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| Keywords: | SSR |
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