SRAP和SSR标记对马铃薯遗传多样性的差异分析

掌握宁夏地区主要马铃薯品种的遗传多样性,了解其遗传背景,明确各品种间的亲缘关系为马铃薯育种提供理论依据。利用SSR和SRAP 2种分子标记对47份马铃薯种质材料进行遗传多样性分析,并对2种分子标记结果进行比较。12对多态性SRAP标记共检测到180个多态性位点,平均每对引物检测到15个多态性位点,每个SRAP位点的PIC值为0.2~0.43,平均值为0.34;47份马铃薯种质资源遗传相似性系数为0.57~0.83。15对多态性SSR标记检测到80条多态性位点,平均每对引物检测到5.3个多态性位点,每个SSR位点的PIC值为0.37~0.72,平均值为0.51;马铃薯种质材料遗传相似性系数为0.60~0.97。根据系谱资料分析发现,SRAP标记更适用于遗传关系较近材料的遗传多样性分析。宁夏地区主要的马铃薯品种遗传相似度较低,亲缘关系较远。

Variance Analysis of Genetic Diversity of Potato with SRAP and SSR Markers

This study was to understand the genetic diversity and genetic background of the main potato varieties in Ningxia, clarify the relationship among varieties, and provide the theoretical basis for potato breeding. The genetic diversity of 47 potato germplasm materials was studied with SSR and SRAP markers, and the results of these two maker systems were compared. The results showed that 180 polymorphic fragments were detected using 12 SRAP primer pairs, with an average number of polymorphic fragments per primer pair of 15. The PIC for SRAPs was from 0.2 to 0.43 with an average of 0.34. The genetic similarity coefficient of the 47 germplasm materials varied from 0.57 to 0.83 by SRAP markers. Whereas, 80 polymorphic fragments were detected using 15 SSR primer pairs, with an average number of polymorphic fragments per primer pair of 5.3. The PIC for SSRs was from 0.37 to 0.72 with an average of 0.51. The genetic similarity coefficient of the 47 germplasm materials varied from 0.60 to 0.97 by SSR markers. According to the pedigree information, the SRAP markers were more effective for genetic analysis of closely related lines. Potato varieties in Ningxia had low genetic similarity and distant genetic relationship.