纤维蛋白原的5种提取方法比较研究

为了确定纤维蛋白原的最佳提取方案。本研究主要采用反复冷沉淀法、经典冷沉淀法、冷乙醇法、硫酸铵沉淀法和聚乙二醇沉淀法对猪抗凝全血进行纤维蛋白原的提取;以单次血液处理量、制备流程耗时、纤维蛋白原的浓度,是否需要添加化学试剂和所得纤维蛋白原的纯度为考察指标,确定纤维蛋白原的最佳提取方案。结果表明：反复冷沉淀法与经典冷沉淀法相比可以明显提高提取的纤维蛋白原浓度,与化学法相比,全程均采用物理方法,无需添加任何化学制剂,不存在化学制剂回收问题,纤维蛋白原纯度较高,提取的纤维蛋白原浓度符合外用纤维蛋白原质量标准。对结果综合分析可知,反复冷沉淀法是最佳的纤维蛋白原的提取方法。

Comparative Study of Five Kinds of Extraction Methods of Fibrinogen

In order to determine the optimal method on fibrinogen extraction, the repeated cold precipitation method, classical cold precipitation method, cold ethanol method, ammonium sulfate precipitation method and polyethylene glycol precipitation method were adopted to extract fibrinogen from pig anticoagulated blood. All methods were assessed according to the blood volume processed, time-consuming and content of fibrinogen obtained in each process, whether additional chemicals need to be added and purity of the product. The results showed that compared with the classical cold precipitation, repeated cold precipitation could significantly improve the fibrinogen concentration. Compared with the chemical method, the whole process is physical, with no need to add additional chemicals, thus do not need further purification. The purity is relatively high and meets the standards of external use of the product. As a conclusion, the repeated cold precipitation method showed the optimal effect in fibrinogen extraction.