不同基因型大麦离体培养的花药及小孢子对低温和NaCl预处理的反应

以探讨大麦单倍体细胞水平的耐冷、耐NaCl性与植株水平抗病、耐盐性的相关性为目的，用赤霉病抗性、耐盐性不同的大麦为供试材料，比较了它们的离体培养花药及小孢子对低温、NaCl预处理的培养反应。结果表明：感病材料低温（5℃）预处理17d的花药培养愈伤组织诱导率比未经低温预处理的低，抗病材料则有一定的升幅。用NaCl溶液直接处理游离小孢子，所有供试材料的小孢子存活率随处理浓度升高不断下降，耐盐材料的存活率下降幅度比盐敏材料的小，且抗病材料的降幅比感病材料的小。说明：供体植株水平的抗病、耐盐性在花药、小孢子水平上也能有所表现。

Response of in Vitro Culture of Anther and Microspore from Different Genotypes of Barley to Cool and Nacl Pretreatments

The purpose of this research is to investigate the relationship between tolerance to disease and salt of plant and tolerance to cold and NaCl of their haploid cell level in barley. Using barley lines with different tolerance to Fusarium head blight (TF) and Salt (TS) as donor materials, the response of in vitro culture of anther and microspores to cold and NaCl pretreatments were compared. The results showed: the frequency of callus induction from anther culture pretreated spikes for 17d with 5℃ was lower than that of anthers without cold pretreatment for lines with lower TF and the frequency of callus induction was higher from the cold pretreatment compared to the 0d treatment for lines with higher TF. The Freq. of survival of microspores was decreased with the increase of NaCl concentration. The decrease was lower for lines with higher TS than that of lines with lower TS and the decrease also lower for line with higher TF compared to that of line with lower TF. From the research, it could be suggested that the difference of tolerance to disease and salt of donor plants can be expressed in their anther and microspore level.