不同基因型香料烟叶片发育过程中碳氮代谢关键酶活性及代谢产物变化

摘 要：研究了浙江新昌不同基因型香料烟叶片发育过程中碳氮代谢关键酶的活性及代谢产物的含量变化规律。结果表明，在香料烟叶片发育过程中，酸性转化酶(INV)的活性均呈下降趋势；谷氨酰胺合成酶（GS）的活性Samsun品种在20d叶龄时最高，而Basma和Canik在30d叶龄时最高；苯丙氨酸解氨酶（PAL）的活性均表现为30d时最低；随着叶片成熟总氮含量降低，烟碱含量升高，总碳、可溶性总糖随着叶片成熟其含量呈升高趋势；品种不同，碳氮代谢关键酶的活性及代谢产物的含量变化均有很大差异。

Variety of Key Enzymes Activeness and Productions of C and N Metabolism during Leaves Development of Various Genotypes Oriental Tobacco

Abstract: The changes on the key enzymes activity in carbon and nitrogen metabolism and metabolites during the development of oriental tobacco leaves with different genotypes in Zhejiang Xinchang were studied. The results show that during the development of the oriental tobacco leaves, the activity of acid invertase(INV) was decreased; the changes of GS reached its peak in the 20dans in Samsun, but in the 30dans in Basma and Canik ;the activity of PAL was decreased in the 30dans; The content of total nitrogen was decreased with the mature leaves, but nicotine、total carbon and soluble total sugar were increased. Different varieties, carbon and nitrogen metabolism key enzyme activity and the content of metabolites were different.